Isolation and characterization of multiple drug resistance bacterial pathogens from waste water in hospital and non-hospital environments, Northwest Ethiopia

BMC Research Notes

Walelegn Worku³  Yeshambel Belyhun¹  Mengistu Endris²  Feleke Moges² 

[1] Institute of Virology, Faculty of Medicine, University of Leipzig, Leipzig, Germany;Department of Medical Microbiology, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia;Department of Environmental and Occupational Health and Safety, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia

关键词: Waste water;    Non-hospital environment;    Multiple drug resistance;    Hospital environment;   

Others  :  1133979
DOI  :  10.1186/1756-0500-7-215

 received in 2013-09-29, accepted in 2014-04-02,  发布年份 2014

【 摘 要 】

Background

The importance of bacterial isolates from waste water environment as a reservoir of antibiotic resistance and a potential source of novel resistance genes to clinical pathogens is underestimated. This study is aimed at to isolate and characterize public health important bacteria from waste water in hospital and non- hospital environments and evaluate the distribution of multiple drug resistance bacteria in the study area.

Methods

A cross-sectional study was conducted at Gondar from January-June 2012. The hospital waste water was taken from different sections of the Gondar University Teaching Hospital. Non- hospital environment samples were taken at different sites of the university campuses, Gondar College of Teachers education, and soft drink factory in Gondar. Samples were aseptically collected, transported and processed with in two hours following standard procedure. Identified organisms were assessed for different antibiotics following Kirby-Bauer disk diffusion method. All data was registered and entered in to SPSS version 16 computer program. P-values less than 0.05 were taken as statistically significant.

Result

A total of 60 waste water samples were processed for the presence of drug resistance pathogens. Among the total samples 113 bacterial isolates were recovered and of these 65 (57.5%) were from hospital environment and 48 (42.5%) were from non-hospital environment. The most frequently identified bacterium was Klebsiella spp. 30 (26.6%) followed by Pseudomonas spp. 19(16.8%), E. coli (11.5%) and Citrobacter spp (11.5%), and Staphylococcus aureus (8.2%). The over all prevalence of multiple drug resistance (MDR) in this study was 79/113 (69.9%). MDR in hospital environment was found to be 53/68 (81.5%) while in non hospital environment was found to be 26/48 (54.2%).

Conclusions

Multiple drug resistance to the commonly used antibiotics is high in the study area. The contamination of waste water by antibiotics or other pollutants lead to the rise of resistance due to selection pressure. The presence of antibiotic resistance organisms in this waste water should not be overlooked. Since this organisms may be vital to the safety and well-being of patients who are hospitalized and individual susceptible to infection. Therefore, proper waste water treatment plant should be established and improved sanitary measure should be practice.

【 授权许可】

   
2014 Moges et al.; licensee BioMed Central Ltd.

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