Genome and transcriptome sequencing identifies breeding targets in the orphan crop tef (Eragrostis tef)

BMC Genomics

Zerihun Tadele¹  Kebebew Assefa⁶  Cris Kuhlemeier¹  Laurent Falquet⁷  Michel Schneider⁵  Eric Lyons⁸  Laurent Farinelli⁹  Regula Blösch¹  Solomon Chanyalew⁶  Edouard de Castro⁵  Dejene Girma²  Yi Song Wilson¹  Stéphanie Larti⁴  Korinna Esfeld¹  Sonia Plaza-Wüthrich¹  Gina Cannarozzi³ 

[1] University of Bern, Institute of Plant Sciences, Altenbergrain 21, Bern CH-3013, Switzerland;Ethiopian Institute of Agricultural Research, National Biotechnology Laboratory (Holetta), P.O. Box 2003, Addis Ababa, Ethiopia;Swiss Institute of Bioinformatics, Vital-IT, Quartier Sorge - Batiment Genopode, Lausanne 1015, Switzerland;Current address: University of Bern, Clinic for Parodontology, Freiburgstrasse 7, Bern CH-3010, Switzerland;Swiss Institute of Bioinformatics, Rue Michel-Servet 1, 1211, Geneva 4, Switzerland;Ethiopian Institute of Agricultural Research, Debre Zeit Agricultural Research Center, P.O. Box 32, Debre Zeit, Ethiopia;Current address: University of Fribourg, Faculty of Science, Ch. du Musée 10, Fribourg CH-1700, Switzerland;Univerisity of Arizona, School of Plant Sciences, 1140 E. South Campus Drive, 303 Forbes Building, P.O. Box 210036, Tucson, AZ 85721-0036, USA;Fasteris SA, Ch. du Pont-du-Centenaire 109, P.O. Box 28, Plan-les-Ouates CH-1228, Switzerland

关键词: Prolamin;    Abiotic stress;    Transcriptome;    Genome;    Eragrostis tef;    Tef;   

Others  :  1216495
DOI  :  10.1186/1471-2164-15-581

 received in 2014-03-27, accepted in 2014-07-03,  发布年份 2014

【 摘 要 】

Background

Tef (Eragrostis tef), an indigenous cereal critical to food security in the Horn of Africa, is rich in minerals and protein, resistant to many biotic and abiotic stresses and safe for diabetics as well as sufferers of immune reactions to wheat gluten. We present the genome of tef, the first species in the grass subfamily Chloridoideae and the first allotetraploid assembled de novo. We sequenced the tef genome for marker-assisted breeding, to shed light on the molecular mechanisms conferring tef’s desirable nutritional and agronomic properties, and to make its genome publicly available as a community resource.

Results

The draft genome contains 672 Mbp representing 87% of the genome size estimated from flow cytometry. We also sequenced two transcriptomes, one from a normalized RNA library and another from unnormalized RNASeq data. The normalized RNA library revealed around 38000 transcripts that were then annotated by the SwissProt group. The CoGe comparative genomics platform was used to compare the tef genome to other genomes, notably sorghum. Scaffolds comprising approximately half of the genome size were ordered by syntenic alignment to sorghum producing tef pseudo-chromosomes, which were sorted into A and B genomes as well as compared to the genetic map of tef. The draft genome was used to identify novel SSR markers, investigate target genes for abiotic stress resistance studies, and understand the evolution of the prolamin family of proteins that are responsible for the immune response to gluten.

Conclusions

It is highly plausible that breeding targets previously identified in other cereal crops will also be valuable breeding targets in tef. The draft genome and transcriptome will be of great use for identifying these targets for genetic improvement of this orphan crop that is vital for feeding 50 million people in the Horn of Africa.

【 授权许可】

   
2014 Cannarozzi et al.; licensee BioMed Central Ltd.

【 图 表 】

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