Evaluation of antinociceptive effect of methanolic extract of leaves of Crataeva nurvala Buch.-Ham.

BMC Complementary and Alternative Medicine

Mohammad Zafar Imam²  Md Moniruzzaman¹ 

[1] College of Pharmacy, Dongguk University, Goyang 410-820, Republic of Korea;Department of Pharmacy, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh

关键词: Medicinal plants;    Glutamatergic system;    Opioid system;    Capparidaceae;    Crataeva nurvala;    Antinociceptive;   

Others  :  1086568
DOI  :  10.1186/1472-6882-14-354

 received in 2014-05-16, accepted in 2014-09-19,  发布年份 2014

【 摘 要 】

Background

Crataeva nurvala Buch.-Ham. (Family: Capparidaceae) is widely used as anti-inflammatory, contraceptive, laxative, lithotropic, febrifuge and as tonic in traditional medicine. This study evaluated the antinociceptive effect of the methanolic extract of the leaves of Crataeva nurvala (MECN).

Methods

The antinociceptive activity was investigated using heat-induced (hot-plate and tail-immersion test) and chemical-induced (acetic acid, formalin and glutamic acid) nociception models in mice at different doses (50, 100, and 200 mg/kg, p.o.) of MECN. Morphine sulphate (5 mg/kg, i.p.) and diclofenac sodium (10 mg/kg, i. p.) were used as reference analgesic drugs.

Results

MECN produced significant dose-dependent antinociception when assessed using hot plate test, tail immersion test and acetic acid-induced abdominal writhing test (65.55%). Likewise, MECN at similar doses produced significant dose-dependent inhibition in both neurogenic (50.82%) and inflammatory pain (73.53%) induced by intraplantar injection of formalin (2.5% formalin, 20 μl/paw). Besides, MECN also significantly inhibited the glutamate-induced (10 μM/paw) pain in mice (74.68%). It was demonstrated that pretreatment with naloxone (2 mg/kg, i.p.) significantly reversed antinociception produced by MECN in hot plate and tail immersion test suggesting the involvement of opioid receptor. In addition, administration of glibenclamide (10 mg/kg, i.p.), an ATP-sensitive K⁺ channel antagonist could not reverse antinociceptive activity induced by MECN.

Conclusion

The results suggest that MECN possesses antinociceptive activity involving inhibition of opioid system as well as the glutamatergic system supporting its traditional uses.

【 授权许可】

   
2014 Moniruzzaman and Imam; licensee BioMed Central Ltd.

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