Evaluation of antinociceptive effect of methanolic extract of leaves of Crataeva nurvala Buch.-Ham.

BMC Complementary and Alternative Medicine

Research Article

Md Moniruzzaman¹  Mohammad Zafar Imam² 

[1] College of Pharmacy, Dongguk University, 410-820, Goyang, Republic of Korea;Department of Pharmacy, Stamford University Bangladesh, 51 Siddeswari Road, 1217, Dhaka, Bangladesh;

关键词: Antinociceptive;    Crataeva nurvala;    Capparidaceae;    Opioid system;    Glutamatergic system;    Medicinal plants;   

DOI  :  10.1186/1472-6882-14-354

 received in 2014-05-16, accepted in 2014-09-19,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundCrataeva nurvala Buch.-Ham. (Family: Capparidaceae) is widely used as anti-inflammatory, contraceptive, laxative, lithotropic, febrifuge and as tonic in traditional medicine. This study evaluated the antinociceptive effect of the methanolic extract of the leaves of Crataeva nurvala (MECN).MethodsThe antinociceptive activity was investigated using heat-induced (hot-plate and tail-immersion test) and chemical-induced (acetic acid, formalin and glutamic acid) nociception models in mice at different doses (50, 100, and 200 mg/kg, p.o.) of MECN. Morphine sulphate (5 mg/kg, i.p.) and diclofenac sodium (10 mg/kg, i. p.) were used as reference analgesic drugs.ResultsMECN produced significant dose-dependent antinociception when assessed using hot plate test, tail immersion test and acetic acid-induced abdominal writhing test (65.55%). Likewise, MECN at similar doses produced significant dose-dependent inhibition in both neurogenic (50.82%) and inflammatory pain (73.53%) induced by intraplantar injection of formalin (2.5% formalin, 20 μl/paw). Besides, MECN also significantly inhibited the glutamate-induced (10 μM/paw) pain in mice (74.68%). It was demonstrated that pretreatment with naloxone (2 mg/kg, i.p.) significantly reversed antinociception produced by MECN in hot plate and tail immersion test suggesting the involvement of opioid receptor. In addition, administration of glibenclamide (10 mg/kg, i.p.), an ATP-sensitive K+ channel antagonist could not reverse antinociceptive activity induced by MECN.ConclusionThe results suggest that MECN possesses antinociceptive activity involving inhibition of opioid system as well as the glutamatergic system supporting its traditional uses.

【 授权许可】

CC BY   
© Moniruzzaman and Imam; licensee BioMed Central Ltd. 2014