Comparative analysis of the Geobacillus hemicellulose utilization locus reveals a highly variable target for improved hemicellulolysis

BMC Genomics

Don A Cowan²  David A Mead¹  Phillip J Brumm¹  Pieter De Maayer³ 

[1] C5-6 Technologies Inc., Lucigen Corp., 53562 Middleton, WI, USA;Centre for Microbial Ecology and Genomics, Genomics Research Institute, Natural Sciences II Building, University of Pretoria, Pretoria 0002, South Africa;Department of Microbiology and Plant Pathology, University of Pretoria, Pretoria 0002, South Africa

关键词: Arabinofuranose;    Acetylesterase;    Xylanase;    Arabinan;    Arabinoxylan;    Hemicellulose;    Geobacillus;   

Others  :  1139342
DOI  :  10.1186/1471-2164-15-836

 received in 2014-06-02, accepted in 2014-09-23,  发布年份 2014

【 摘 要 】

Background

Members of the thermophilic genus Geobacillus can grow at high temperatures and produce a battery of thermostable hemicellulose hydrolytic enzymes, making them ideal candidates for the bioconversion of biomass to value-added products. To date the molecular determinants for hemicellulose degradation and utilization have only been identified and partially characterized in one strain, namely Geobacillus stearothermophilus T-6, where they are clustered in a single genetic locus.

Results

Using the G. stearothermophilus T-6 hemicellulose utilization locus as genetic marker, orthologous hemicellulose utilization (HUS) loci were identified in the complete and partial genomes of 17/24 Geobacillus strains. These HUS loci are localized on a common genomic island. Comparative analyses of these loci revealed extensive variability among the Geobacillus hemicellulose utilization systems, with only seven out of 41–68 proteins encoded on these loci conserved among the HUS⁺ strains. This translates into extensive differences in the hydrolytic enzymes, transport systems and metabolic pathways employed by Geobacillus spp. to degrade and utilize hemicellulose polymers.

Conclusions

The genetic variability among the Geobacillus HUS loci implies that they have variable capacities to degrade hemicellulose polymers, or that they may degrade distinct polymers, as are found in different plant species and tissues. The data from this study can serve as a basis for the genetic engineering of a Geobacillus strain(s) with an improved capacity to degrade and utilize hemicellulose.

【 授权许可】

   
2014 De Maayer et al.; licensee BioMed Central Ltd.

【 图 表 】

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