Antibody-based screening of cell wall matrix glycans in ferns reveals taxon, tissue and cell-type specific distribution patterns

BMC Plant Biology

Research Article

J Paul Knox¹  Susan E Marcus¹  William GT Willats²  Iben Sørensen³  Ronnie LL Viane⁴  Olivier Leroux⁴ 

[1] Centre for Plant Sciences, Faculty of Biological Sciences, University of Leeds, LS2 9JT, Leeds, UK;Department of Plant Biology and Biotechnology, Copenhagen University, Thorvaldsensvej 40, 1871, Frederiksberg, Denmark;Department of Plant Biology and Biotechnology, Copenhagen University, Thorvaldsensvej 40, 1871, Frederiksberg, Denmark;Department of Plant Biology, Cornell University, 14853, Ithaca, NY, USA;Pteridology, Department of Biology, Ghent University, K.L. Ledeganckstraat 35, B-9000, Ghent, Belgium;

关键词: Cell wall evolution;    Homogalacturonan;    Arabinan;    Galactan;    Xyloglucan;    Xylan;    Mannan;    Mixed-linkage glucan;    Sclerenchyma;   

DOI  :  10.1186/s12870-014-0362-8

 received in 2014-09-16, accepted in 2014-12-01,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundWhile it is kno3wn that complex tissues with specialized functions emerged during land plant evolution, it is not clear how cell wall polymers and their structural variants are associated with specific tissues or cell types. Moreover, due to the economic importance of many flowering plants, ferns have been largely neglected in cell wall comparative studies.ResultsTo explore fern cell wall diversity sets of monoclonal antibodies directed to matrix glycans of angiosperm cell walls have been used in glycan microarray and in situ analyses with 76 fern species and four species of lycophytes. All major matrix glycans were present as indicated by epitope detection with some variations in abundance. Pectic HG epitopes were of low abundance in lycophytes and the CCRC-M1 fucosylated xyloglucan epitope was largely absent from the Aspleniaceae. The LM15 XXXG epitope was detected widely across the ferns and specifically associated with phloem cell walls and similarly the LM11 xylan epitope was associated with xylem cell walls. The LM5 galactan and LM6 arabinan epitopes, linked to pectic supramolecules in angiosperms, were associated with vascular structures with only limited detection in ground tissues. Mannan epitopes were found to be associated with the development of mechanical tissues. We provided the first evidence for the presence of MLG in leptosporangiate ferns.ConclusionsThe data sets indicate that cell wall diversity in land plants is multifaceted and that matrix glycan epitopes display complex spatio-temporal and phylogenetic distribution patterns that are likely to relate to the evolution of land plant body plans.

【 授权许可】

CC BY   
© Leroux et al.; licensee BioMed Central. 2015