Influence of osteogenic stimulation and VEGF treatment on in vivo bone formation in hMSC-seeded cancellous bone scaffolds

BMC Musculoskeletal Disorders

Matthias Schieker³  Denitsa Docheva³  Stefan Milz⁵  Christina Ern⁶  Sebastian Seitz⁴  Florian Pohlig¹  Ulrich Lenze² 

[1] Department of Trauma Surgery, Technical University of Munich (TU), Munich, Germany;Department of Orthopedics and Orthopedic Sports Medicine, Technical University of Munich (TU), Munich, Germany;Laboratory of Experimental Surgery and Regenerative Medicine, Department of Surgery, University of Munich (LMU), Munich, Germany;Department of Orthopaedics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany;Department of Anatomy, University of Munich (LMU), Munich, Germany;Department of Restorative Dentistry & Periodontology, University of Munich (LMU), Munich, Germany

关键词: Osteogenic stimulation;    Vascularization;    VEGF;    Tissue engineering;    hMSC;   

Others  :  1118641
DOI  :  10.1186/1471-2474-15-350

 received in 2014-02-10, accepted in 2014-09-23,  发布年份 2014

【 摘 要 】

Background

Tissue engineering approaches for reconstruction of large bone defects are still technically immature, especially in regard to sufficient blood supply. Therefore, the aim of the present study was to investigate the influence of osteogenic stimulation and treatment with VEGF on new bone formation and neovascularization in hMSC-loaded cancellous bone scaffolds in vivo.

Methods

Cubic scaffolds were seeded with hMSC and either cultured in stem cell medium or osteogenic stimulation medium. One osteogenically stimulated group was additionally treated with 0.8 μg VEGF prior to subcutaneous implantation in athymic mice. After 2 and 12 weeks in vivo, constructs and selected organs were harvested for histological and molecular analysis.

Results

Histological analysis revealed similar vascularization of the constructs with and without VEGF treatment and absence of new bone formation in any group. Human DNA was detected in all inoculated scaffolds, but a significant decrease in cells was observed after 2 weeks with no further decrease after 12 weeks in vivo.

Conclusion

Under the chosen conditions, osteogenic stimulation and treatment with VEGF does not have any influence on the new bone formation and neovascularization in hMSC-seeded cancellous bone scaffolds.

【 授权许可】

   
2014 Lenze et al.; licensee BioMed Central Ltd.

【 图 表 】

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