Animal model of Mycoplasma fermentans respiratory infection

BMC Research Notes

Lilia Cedillo²  Constantino Gil³  Silvia Giono⁴  José Antonio Rivera-Tapia³  Francisco Javier González-Matus¹  Teresa Calixto¹  Azucena Martínez-Ramos¹  Antonio Yáñez¹ 

[1] Laboratorio de Investigación en Microbiología Oral, Facultad de Estomatología, Benemérita Universidad Autónoma de Puebla, 31 Poniente 1304, Col. Volcanes, Puebla, Pue, 72410, México;Centro de Detección Biomolecular, Benemérita universidad Autónoma de Puebla, Boulevard Valsequillo s/n. Ciudad Universitaria, Col. San Manuel, Puebla, Pue, 72570, México;Laboratorio de Micoplasmas. Centro de Investigaciones en Ciencias Microbiológicas del Instituto de Ciencias de la Benemérita Universidad Autónoma de Puebla, Edificio 103-J, Ciudad Universitaria, Col. San Manuel, Puebla, Pue, 72570, México;Laboratorio de Bacteriología Médica. Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n. Col. Santo Tomás, México, D.F. 11340, México

关键词: Respiratory infection;    Mycoplasma fermentans;    Mycoplasma;    Animal model;   

Others  :  1145046
DOI  :  10.1186/1756-0500-6-9

 received in 2012-12-08, accepted in 2012-12-21,  发布年份 2013

【 摘 要 】

Background

Mycoplasma fermentans has been associated with respiratory, genitourinary tract infections and rheumatoid diseases but its role as pathogen is controversial. The purpose of this study was to probe that Mycoplasma fermentans is able to produce respiratory tract infection and migrate to several organs on an experimental infection model in hamsters. One hundred and twenty six hamsters were divided in six groups (A-F) of 21 hamsters each. Animals of groups A, B, C were intratracheally injected with one of the mycoplasma strains: Mycoplasma fermentans P 140 (wild strain), Mycoplasma fermentans PG 18 (type strain) or Mycoplasma pneumoniae Eaton strain. Groups D, E, F were the negative, media, and sham controls. Fragments of trachea, lungs, kidney, heart, brain and spleen were cultured and used for the histopathological study. U frequency test was used to compare recovery of mycoplasmas from organs.

Results

Mycoplasmas were detected by culture and PCR. The three mycoplasma strains induced an interstitial pneumonia; they also migrated to several organs and persisted there for at least 50 days. Mycoplasma fermentans P 140 induced a more severe damage in lungs than Mycoplasma fermentans PG 18. Mycoplasma pneumoniae produced severe damage in lungs and renal damage.

Conclusions

Mycoplasma fermentans induced a respiratory tract infection and persisted in different organs for several weeks in hamsters. This finding may help to explain the ability of Mycoplasma fermentans to induce pneumonia and chronic infectious diseases in humans.

【 授权许可】

   
2013 Yañez et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

【 参考文献 】

• [1]Baseman JB, Tully JG: Mycoplasmas: sophisticated, reemerging and burdened by their notoriety. Emerg Infect Dis 1997, 3(1):21-32.
• [2]Williams MH, Brostoff J, Roitt IM: Possible role of Mycoplasma fermentans in pathogenesis of rheumatoid arthritis. Lancet 1970, 296(7667):277-280.
• [3]Kovacic R, Launay V, Tuppin P, Lafeuillade A, Feuillie V, Montagnier L, Grau O: Search for the presence of six Mycoplasma species in peripheral blood mononuclear cells of subjects seropositive and seronegative for human immunodeficiency virus. J Clin Microbiol 1996, 34(7):1808-1810.
• [4]Katseni VL, Gilroy CB, Ryait BK, Ariyoshi K, Bieniasz PD, Weber JN, Taylor-Robinson D: Mycoplasma fermentans in individuals seropositive and seronegative for HIV-1. Lancet 1993, 341:271-273.
• [5]Barile MF, Chandler DKF, Yoshida H, Grabowaki MW, Harasawa R, Razin S: Parameters of Mycoplasma pneumoniae infection in Syrian Hamsters. Infect Immun 1988, 56(9):2443-2449.
• [6]Vojdani A, Choppa PC, Tagle C, Andrin R, Samimi B, Lapp CW: Detection of Mycoplasma genus and Mycoplasma fermentans by PCR in patients with chronic fatigue syndrome. FEMS Immunol Med Microbiol 1998, 22(4):355-365.
• [7]van Kuppeveld FJ, van der Logt JT, Angulo AF, van Zoest MJ, Quint WG, Niesters HG, Galama JM, Melchers WJ: Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification. Appl Envir Microbiol 1992, 58:2606-2615.
• [8]Bernet C, Garret M, De Barbeyrac B, Bebear C, Bonnet J: Detection of Mycoplasma pneumoniae by using the polymerase chain reaction. J Clin Microbiol 1989, 27(11):2492-2496.
• [9]Cassell GH, Yañez A, Duffy L, Moyer J, Cedillo L, Hammerschlag MR, Rank RG, Glass JI: Detection of Mycoplasma fermentans in the respiratory tract of children with pneumonia [abstract]. IOM Letters 1999, 3:456.
• [10]Haier J, Nasralla M, Franco AR, Nicolson GL: Detection of mycoplasmal infections in blood of patients with rheumatoid arthritis. Rheumatology 1999, 38:504-509.
• [11]Rechnitzer H, Brzuszkiewicz E, Strittmatter A, Liesegang H, Lysnyansky I, Daniel R, Gottschalk G, Rottem S: Genomic features and insights into the biology of Mycoplasma fermentans. Microbiology 2011, 157:760-773.
• [12]Schaeverbeke T, Clerc M, Lequen L, Charron A, Bébéar C, Barbeyrac B, Bannwarth B, Dehais J, Bébéar C: Genotypic characterization of seven strains of Mycoplasma fermentans isolated from synovial fluids of patients with arthritis. J Clin Microbiol 1998, 36(5):1226-1231.
• [13]Gilroy CB, Keat A, Taylor-Robinson D: The prevalence of Mycoplasma fermentans in patients with inflammatory arthritides. Rheumatology 2001, 40:1355-1358.
• [14]Kawahito Y, Ichinose S, Sano H, Tsubouchi Y, Kohno M, Yoshikawa T, Tokunaga D, Hojo T, Harasawa R, Nakano T, Matsuda K: Mycoplasma fermentans glycolipid-antigen as a pathogen of rheumatoid arthritis. Biochem Biophys Res Commun 2008, 369:561-566.
• [15]Waites KB, Atkinson TP: The role of Mycoplasma in upper respiratory infections. Current Infect Dis Reports 2009, 11:198-206.
• [16]Neumayr L, Lennette E, Kelly D, Earles A, Embury S, Groncy P, Grossi M, Grover R, McMahon L, Swerdlow P, Waldrom P, Vichinsky E: Mycoplasma disease and acute chest syndrome in sickle cell disease. Pediatrics 2003, 112:87-95.
• [17]Ramírez AS, Rosas A, Hernández-Beriain JA, Orengo JC, Saavedra P, de la Fé C, Fernández A, Poveda JB: Relationship between rheumatoid arthritis and Mycoplasma pneumoniae: a case–control study. Rheumatology 2005, 44:912-914.
• [18]Fabisiak JP, Gao F, Thomson RG, Strieter RM, Watkins S, Dauber JH: Mycoplasma fermentans and TNF- beta interact to amplify immune-modulating cytokines in human lung fibroblasts. Am J Physiol 2006, 291:L781-L793.
• [19]Rottem S: Interaction of mycoplasmas with host cells. Physiol Rev 2003, 83:417-432.
• [20]Zhang S, Tsai S, Wu TT, Li B, Shih JW, Lo S: Mycoplasma fermentans infection promotes immortalization of human peripheral blood mononuclear cells in culture. Blood 2004, 104(13):4252-4259.