A ferritin-like protein with antioxidant activity in Ureaplasma urealyticum

BMC Microbiology

Yimou Wu³  Xiaoxing You³  Chuanhao Jiang²  Hongliang Chen¹  Ranhui Li³  Guozhi Dai¹ 

[1] Department of Clinical Laboratory, The First People’s Hospital of Chenzhou, Chenzhou 42300, Hunan, China;Institute of Pathogenic Biology, University of South China, Changsheng road 28, Hengyang 421001, Hunan, China;Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang 421001, Hunan, China

关键词: Antioxidant activity;    Reactive oxygen species;    Ferritin;    Ureaplasma urealyticum;   

Others  :  1227618
DOI  :  10.1186/s12866-015-0485-6

 received in 2014-12-22, accepted in 2015-07-13,  发布年份 2015

【 摘 要 】

Background

Ureaplasma urealyticum is a major pathogen associated with many diseases. The ability of U. urealyticum to protect itself from oxidative stress is likely to be important for its pathogenesis and survival, but its oxidative stress tolerance mechanisms remain unclear. This study investigates the antioxidant activity of a ferritin-like protein from U. urealyticum.

Results

The uuferritin gene, which was up regulated when U. urealyticum was subjected to oxidative stress, was cloned from U. urealyticum and the corresponding recombinant protein uuferritin was purified. Uuferritin protein reduced the levels of hydroxyl radicals generated by the Fenton reaction as a consequence of its ferroxidase activity, and thus the protein protected DNA from oxidative damage. Furthermore, oxidation-sensitive Escherichia coli mutants transformed with pTrc99a-uuferritin showed significantly improved tolerance to oxidative stress compared to E. coli mutants transformed with an empty pTrc99a vector.

Conclusions

The present work shows that uuferritin protein confers resistance to oxidative stress in vitro and in E. coli. The protective role of uuferritin provides a foundation for understanding the mechanisms of oxidative stress tolerance in U. urealyticum.

【 授权许可】

   
2015 Dai et al.

【 图 表 】

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