Rapid determination of flavonoids and phenolic acids in grape juices and wines by RP-HPLC/DAD: Method validation and characterization of commercial products of the new Brazilian varieties of grape

卷:228

da Silva Padilha, Carla Valeria ; Miskinis, Gabriela Aquino ; Alves Olinda de Souza, Marcelo Eduardo ; Pereira, Giuliano Elias ; de Oliveira, Debora ; Bordignon-Luiz, Marilde Terezinha ; Lima, Marcos dos Santos

Inst Fed Sertao Pernambucano

关键词: Bioactive compounds;    Antioxidant activity;    Vitis labrusca L.;    Reactive oxygen species;   

DOI  :  10.1016/j.foodchem.2017.01.137

学科分类：食品科学和技术

【 摘 要 】

A method for rapid determination of phenolic compounds by reversed-phase high-performance liquid chromatography (RP-HPLC), using a new column of faster resolution was validated and used to characterize commercial products produced with new grape Brazilian varieties of Northeast of Brazil. The in vitro antioxidant activity was also measured. The method showed linearity (R > 0.9995), good precision (CV% < 2.78), recovery (91.8-105.1%) and limits of detection (0.04-0.85 mg L-1) and quantification (0.04-1.41 mg L-1) according to other methods previously published with the difference of a run time of only 25 min. The results obtained in the characterization of the samples differed for juices and wines from other world regions, mainly because of the high values of (-)-epigallocatechin and trans-caftaric acid. The products analyzed showed high antioxidant activity, especially the wine samples with values higher than those from wines of different regions of the world. (C) 2017 Elsevier Ltd. All rights reserved.