Expression of phosphatase of regenerating liver family genes during embryogenesis: an evolutionary developmental analysis among Drosophila, amphioxus, and zebrafish

BMC Developmental Biology

Yau-Hung Chen⁵  Jr-Kai Yu⁴  Min-Lang Huang¹  Yu-Di Chang²  Kai-Wen Cheng⁵  Hsin-Lun Hsien²  Han-Ru Li⁴  Szu-Chieh Wang³  Hsun-Tzu Lee⁵  Ming-Der Lin² 

[1] Department of Life Science, National Chung-Cheng University, No.168, Sec. 1, University Rd, Min-Hsiung Township, Chia-yi County 621, Taiwan;Department of Life Sciences, Tzu-Chi University, No.701, Zhongyang Rd, Sec 3, Hualien 97004, Taiwan;Department of Molecular Biology and Human Genetics, Tzu-Chi University, No.701, Zhongyang Rd., Sec 3, Hualien 97004, Taiwan;Institute of Cellular and Organismic Biology, Academia Sinica, 128 Academia Road, Sec. 2, Nankang, Taipei 11529, Taiwan;Department of Chemistry, Tamkang University, 151 Ying-Chuan Rd., Tamsui, New Taipei City 25137, Taiwan

关键词: Amphioxus;    Zebrafish;    Drosophila;    Embryogenesis;    PTP4A;    Phosphatase of regenerating liver;   

Others  :  1085816
DOI  :  10.1186/1471-213X-13-18

 received in 2013-01-25, accepted in 2013-04-29,  发布年份 2013

【 摘 要 】

Background

Phosphatase of regenerating liver (PRL) family is classified as class IVa of protein tyrosine phosphatase (PTP4A) that removes phosphate groups from phosphorylated tyrosine residues on proteins. PRL phosphatases have been implicated in a number of tumorigenesis and metastasis processes and are highly conserved. However, the understanding of PRL expression profiles during embryonic development is very limited.

Results

In this study, we demonstrated and characterized the comprehensive expression pattern of Drosophila PRL, amphioxus PRL, and zebrafish PRLs during embryonic development by either whole mount immunostaining or in situ hybridization. Our results indicate that Drosophila PRL is mainly enriched in developing mid-guts and central nervous system (CNS) in embryogenesis. In amphioxus, initially PRL gene is expressed ubiquitously during early embryogenesis, but its expression become restricted to the anterior neural tube in the cerebral vesicle. In zebrafish, PRL-1 and PRL-2 share similar expression patterns, most of which are neuronal lineages. In contrast, the expression of zebrafish PRL-3 is more specific and preferential in muscle.

Conclusions

This study, for the first time, elucidated the embryonic expression pattern of Drosophila, amphioxus, and zebrafish PRL genes. The shared PRL expression pattern in the developing CNS among diverse animals suggests that PRL may play conserved roles in these animals for CNS development.

【 授权许可】

   
2013 Lin et al.; licensee BioMed Central Ltd.

【 图 表 】

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