Jaagsiekte sheep retrovirus detected in human lung cancer tissue arrays

BMC Research Notes

Sarah K Wootton²  Carl Morrison¹  Paul N Bogner¹  Scott R Walsh²  Nicolle M Linnerth-Petrik² 

[1] Department of Pathology, Roswell Park Cancer Institute, Elm & Carlton Streets, Buffalo 14263, New York, USA;Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1, Canada

关键词: Tissue microarray;    PCR;    Immunohistochemistry;    Jaagsiekte sheep retrovirus;    Ovine pulmonary adenocarcinoma;    Bronchioloalveolar carcinoma;    Lung cancer;   

Others  :  1134226
DOI  :  10.1186/1756-0500-7-160

 received in 2013-06-03, accepted in 2014-03-13,  发布年份 2014

【 摘 要 】

Background

Adenocarcinoma is the most common type of non-small cell lung cancer and is frequently observed in non-smoking patients. Adenocarcinoma in-situ (formerly referred to as bronchioloalveolar carcinoma) is a subset of lung adenocarcinoma characterized by growth along alveolar septae without evidence of stromal, vascular, or pleural invasion, that disproportionately affects never-smokers, women, and Asians. Adenocarcinoma in-situ is morphologically and histologically similar to a contagious lung neoplasm of sheep called ovine pulmonary adenocarcinoma (OPA). OPA is caused by infection with the exogenous betaretrovirus, jaagsiekte sheep retrovirus (JSRV), whose envelope protein (Env) is a potent oncogene. Several studies have reported that a proportion of human lung adenocarcinomas are immunopositive for an antigen related to the Gag protein of JSRV, however other groups have been unable to verify these observations by PCR.

Methods

Here we examine human lung cancer tissue arrays (TA) for evidence of JSRV Env protein and DNA by immunohistochemical staining and PCR, respectively.

Results

Our results reveal that a subset of human lung cancers express an antigen that reacts with a JSRV Env-specific monoclonal antibody in immunohistochemistry and that exogenous JSRV-like env and gag sequences can be amplified from TA tumor samples, albeit inefficiently.

Conclusions

While a causative role has not been established, these data suggest that a JSRV-like virus might infect humans. With next generation sequencing approaches, a JSRV-like virus in human lung cancers may be identified which could have profound implications for prevention, diagnosis and therapy.

【 授权许可】

   
2014 Linnerth-Petrik et al.; licensee BioMed Central Ltd.

【 图 表 】

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