De novo transcriptome assembly and analysis of Sf21 cells using illumina paired end sequencing

Biology Direct

Pavan Kumar Kakumani²  Rohit Shukla¹  Vivek N. Todur¹  Pawan Malhotra²  Sunil K. Mukherjee³  Raj K. Bhatnagar² 

[1] Bionivid Technology Pvt. Ltd., 401, 4 AB Cross, 1st Main, Kasturi Nagar, NGEF East, Bangalore 560043, India

[2] International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India

[3] Present address: Department of Genetics, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India

关键词: Spodoptera frugiperda;    Sf21 cells;    Pest;    Insect;    Army worm;   

Others  :  1225798
DOI  :  10.1186/s13062-015-0072-7

 received in 2015-05-15, accepted in 2015-08-06,  发布年份 2015

【 摘 要 】

Spodoptera is an important polyphagous agricultural insect pest in the tropical world. The genomic details are limited to understand the pest biology at molecular level. In the present study, we sequenced and assembled the transcriptome from Sf21 cells into a non redundant set of 24,038 contigs of ~ 47.38 Mb in size. A total of 26,390 unigenes were identified from the assembled transcripts and their annotation revealed the prevalent protein domains in Sf21 cells. The present study would provide a resource for gene discovery and development of functional molecular markers to understand the biology of S. frugiperda.

【 授权许可】

   
2015 Kakumani et al.

【 图 表 】

Fig. 1.

Fig. 1.

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