Helical computed tomography scanning of the larynx and upper trachea in rabbits

Acta Veterinaria Scandinavica

Gamal Karrouf¹  Samar Rabah⁶  Osama Marglani⁴  Ameen Alherabi⁴  Hani Marzouki²  Abdulrahman Aljohani²  Abdulrahman Althubaiti²  Alamin Salih³  Saddig Jastaniah³  Mariam Al-Sheikah²  Talal Al-Khatib²  Amr M. Ajlan⁵ 

[1]Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt

[2]Department of Otolaryngology, Head and Neck Surgery, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia

[3]Department of Diagnostic Radiology, Faculty of Applied Medical Science, King Abdulaziz University, Jeddah, Saudi Arabia

[4]Department of Otolaryngology, Head and Neck Surgery, Umm Al-Qura University, Mecca, Saudi Arabia

[5]Radiology Department, King Abdulaziz University Hospital, King Abdulaziz University, Jeddah, Western Region, Saudi Arabia

[6]Department of Biology, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia

关键词: Rabbits;    Subglottis;    Trachea;    Helical computed tomography;   

Others  :  1228602
DOI  :  10.1186/s13028-015-0157-4

 received in 2015-04-09, accepted in 2015-09-23,  发布年份 2015

【 摘 要 】

Background

Computed tomography (CT) is used to evaluate the human tracheobronchial tree because of its unsurpassed ability to visualize the airway and surrounding structures. To establish an ideal animal model for studying subglottic stenosis, we assessed the size and morphology of the normal rabbit’s laryngotracheal airway by helical CT. We measured luminal dimensions at the levels of the arytenoid and cricoid cartilages and the first, third, and eighth tracheal rings. At all levels, the axial slices were used to calculate the maximum anteroposterior (AP) dimension, transverse dimension, and cross-sectional areas. We measured the tracheal length from the cricoid to the third and eighth tracheal rings on sagittal reformation. We assessed the hyoid, thyroid, cricoid, arytenoid, and tracheal rings for the presence of calcific or soft tissue densities. We also addressed the presence or absence of pre-epiglottic and paraglottic fat.

Results

The mean AP tracheal dimension ± standard deviation (SD) was 8.6 ± 0.5 mm at the arytenoid level, 8.2 ± 0.7 mm at the cricoid level, and 7.7 ± 0.2 mm at the first tracheal ring level. The transverse tracheal dimension ±SD was 5.3 ± 0.1 mm at the arytenoid level, 5.5 ± 0.5 mm at the cricoid level, and 6.1 ± 0.6 mm at the first tracheal ring level. The mean tracheal area ±SD was 35.7 ± 2.2 mm ²at the arytenoid level, 35.8 ± 5.1 mm ²at the cricoid level, and 39.2 ± 4.3 mm ²at the first tracheal ring level. The tracheal length ±SD was 10.7 ± 2.3 mm from the cricoid to the third tracheal ring and 19.1 ± 1.14 mm to the eighth tracheal ring. There was complete calcification of the hyoid in all rabbits. Only two rabbits showed complete thyroid, arytenoid, or tracheal ring calcification. The remaining airway components were otherwise either uncalcified or partially calcified. The uvula, epiglottis, aryepiglottic fold, vallecula, piriform sinus, true/false vocal cords, and pre-epiglottic/paraglottic fat were not seen in any rabbit.

Conclusions

Helical CT investigation provides good, highly definitive anatomic details of the larynx and trachea in rabbits. Such results may be used in further evaluation of the normal airway and in cases of subglottic stenosis.

【 授权许可】

   
2015 Ajlan et al.

【 图 表 】

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【 参考文献 】

• [1]Hudgins PA, Siegel J, Jacobs I, Abramowsky CR. The normal pediatric larynx on CT and MR. Am J Neuroradiol. 1997; 18:239-245.
• [2]Marom EM, Goodman PC, McAdams HP. Focal abnormalities of the trachea and main bronchi. Am J Roentgenol. 2001; 176:707-711.
• [3]Marom EM, Goodman PC, McAdams HP. Diffuse abnormalities of the trachea and main bronchi. Am J Roentgenol. 2001; 176:713-717.
• [4]Roh J, Lee Y, Park H. Subglottic wound healing in a new rabbit model of acquired subglottic stenosis. Ann Otol Rhinol Laryngol. 2006; 115:611-616.
• [5]Otteson TD, Sandulache VC, Barsic M, DiSilvio GM, Hebda PA, Dohar JE. Acute and chronic changes in the subglottis induced by graded CO 2 laser injury in the rabbit airway. Arch Otolaryngol Head Neck Surg. 2008; 134:1-9.
• [6]Singh T, Ulache S, Otteson V, Barsic T, Klein M, Dohar E, Hebda JP. Subglottic stenosis (SGS) examined as a fibrotic airway mucosal response to injury characterized by altered mucosal fibroblast activity. Arch Otolaryngol Head Neck Surg. 2010; 136:163-170.
• [7]Kelly N, Murphy M, Giles S, Russell J. Subglottic injury: a clinically relevant animal model. Laryngoscope. 2012; 122:2574-2581.
• [8]Lin J, Yau A, Boyd J, Hamamoto A, Su E, Tracy L et al.. Real-time subglottic stenosis imaging using optical coherence tomography in the rabbit. JAMA Otolaryngol Head Neck Surg. 2013; 139:502-509.
• [9]Loewen M, Walner D. Dimensions of rabbit subglottis and trachea. Lab Anim. 2001; 35:253-256.
• [10]Uzunova K. Hygiene, ethology and animal protection, 320 pages. Publishing House Jemmy Stratus, Stara Zagora, Bulgaria; 2011.
• [11]Dinev D, Simeonova G. Veterinary anesthesiology, 300 pages. Kota Publishing House, Stara Zagora; 2009.
• [12]Walner DL, Cotton RT, Toriumi DM, Willging JP. The effect of platelet derived growth factor in laryngotracheal surgery utilizing a rabbit model. Head Neck Surg. 1996; 9:115-116.
• [13]Zotti A, Banzato T, Cozzi B. Cross-sectional anatomy of the rabbit neck and trunk: comparison of computed tomography and cadaver anatomy. Res Vet Sci. 2009; 87:171-176.
• [14]Griscom NT, Wohl ME. Dimensions of the growing trachea related to age and gender. AJR Am J Roentgenol. 1986; 146:233-337.
• [15]Fishman E, Horton K. The increasing impact of multidetector computed tomography in clinical practice. Eur J Radiol. 2007; 62:1-13.