Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffolds

Acta Cirurgica Brasileira

Thaiane Cristine Evaristo¹  Flávia Cilene Maciel Da Cruzalves¹  Andrei Moroz¹  Woner Mion¹  Michele Janegitz Acorci-valério¹  Sérgio Luis Felisbino¹  Rosana Rossi-ferreira¹  Raul Lopes Ruiz Júnior¹  Elenice Deffune¹ 

关键词: Tissue Engineering;    Biocompatible Materials;    Quantum Dots;    Trachea;    Rabbits;   

DOI  :  10.1590/S0102-86502014000800002

来源: SciELO

【 摘 要 】

PURPOSE: To obtain a decellularized tracheal scaffold associating traditional approaches with the novel light-emitting diode (LED) proposal.METHODS:This study was performed with New Zealand adult rabbits weighing 3.0 - 4.0 kg. Different protocols (22) were used combining physical (agitation and LED irradiation), chemical (SDS and Triton X-100 detergents), and enzymatic methods (DNase and RNase).RESULTS:Generally, the cells surrounding soft tissues were successfully removed, but none protocol removed cells from the tracheal cartilage. However, longer protocols were more effective. The cost-benefits relation of the enzymatic processes was not favorable. It was possible to find out that the cartilaginous tissue submitted to the irradiation with LED 630nm and 475 nm showed an increased number of gaps without cells, but several cells were observed to be still present.CONCLUSION:The light-emitting diode is a promising tool for decellularization of soft tissues.

【 授权许可】

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