Engraftment of human adipose derived stem cells delivered in a hyaluronic acid preparation in mice

Acta Cirurgica Brasileira

Isa Dietrich²  Olivia Cochet¹  Phi Villageois¹  Consuelo Junqueira Rodrigues¹ 

[1] ,Sao Paulo University School of Medicine Department of Surgery

关键词: Adult Stem Cells;    Tissue Engineering;    Cell Transplantation;    Adipose Tissue;    Hyaluronic Acid;    Biocompatible Materials;    Mice;    Células-Tronco Adultas;    Engenharia Tecidual;    Transplante de Células;    Tecido Adiposo;    Ácido Hialurônico;    Materiais Biocompatíveis;    Camundongos;   

DOI  :  10.1590/S0102-86502012000400001

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate the implant of human adipose derived stem cells (ADSC) delivered in hyaluronic acid gel (HA), injected in the subcutaneous of athymic mice. METHODS: Control implants -HA plus culture media was injected in the subcutaneous of the left sub scapular area of 12 athymic mice. ADSC implants: HA plus ADSC suspended in culture media was injected in the subcutaneous, at the contra lateral area, of the same animals. With eight weeks, animals were sacrificed and the recovered implants were processed for extraction of genomic DNA, and histological study by hematoxilin-eosin staining and immunufluorescence using anti human vimentin and anti von Willebrand factor antibodies. RESULTS: Controls: Not visualized at the injection site. An amorphous substance was observed in hematoxilin-eosin stained sections. Human vimentin and anti von Willebrand factor were not detected. No human DNA was detected. ADSC implants - A plug was visible at the site of injection. Fusiform cells were observed in sections stained by hematoxilin- eosin and both human vimentin and anti von Willebrand factor were detected by immunofluorescence. The presence of human DNA was confirmed. CONCLUSION: The delivery of human adipose derived stem cells in preparations of hyaluronic acid assured cells engraftment at the site of injection.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License