Characterization of human adipose-derived stem cells

Acta Cirurgica Brasileira

Silvana Gaiba¹  Lucimar Pereira De França¹  Jerônimo Pereira De França¹  Lydia Masako Ferreira² 

[1] ,UNIFESP Chairwoman Plastic Surgery Division Head of Department of SurgerySao Paulo SP ,Brazil

关键词: Adipocytes;    Adult Stem Cells;    Mesenchymal Stem Cells;    Stem Cells;    Adipose Tissue;    Adipócitos;    Células-Tronco Adultas;    Células-Tronco Mesenquimais;    Células-Tronco;    Tecido Adiposo;   

DOI  :  10.1590/S0102-86502012000700007

来源: SciELO

【 摘 要 】

PURPOSE: There is a growing scientific interest in the plasticity and therapeutic potential of adipose-derived stem cells (ASCs), which are multipotent and abundant in adipose tissue and can differentiate in vitro into multiple lineages, including adipocytes, chondrocytes, osteoblasts, neural cells, endothelial cells and cardiomyocytes. The aim of this study was to isolate, cultivate and identify ASCs. METHODS: Human adipose precursor cells were obtained from subcutaneous abdominal tissue. Recently dispersed cells were separated by density centrifugation gradient, cultured and then analyzed. RESULTS: Human ASCs were able to replicate in our culture conditions. The cells maintained their phenotypes throughout the studied period on different passages confirming they suitability for in vitro cultivation. We also induced their adipogenic, osteogenic and chondrogenic differentiation, verifying their mesenchymal stem cells potentiality in vitro. Flow cytometry results showed that these cells expressed CD73, CD90 and CD105, (mesenchymal stem-cells markers), contrasting with the lack of expression of CD16, CD34 and CD45 (hematopoietic cells markers). CONCLUSION: It was possible to isolate human adipose-derived stem cells by in vitro cultivation without adipogenic induction, maintaining their functional integrity and high proliferation levels. The cells demonstrated adipogenic, osteogenic and chondrogenic differentiation potential in vitro.

【 授权许可】

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