期刊论文

【摘要】

Background

The C. bescii genome does not encode an acetaldehyde/alcohol dehydrogenase or an acetaldehyde dehydrogenase and no ethanol production is detected in this strain. The recent introduction of an NADH-dependent AdhE from C. thermocellum (Fig. 1a) in an ldh mutant of this strain resulted in production of ethanol from un-pretreated switchgrass, but the thermolability of the C. thermocellum AdhE at the optimum growth temperature of C. bescii (78 °C) meant that ethanol was not produced above 65 °C.

Fig. 1. Proposed scheme for the pyruvate to ethanol pathway in C. thermocellum and T. pseudethanolicus 39E. a The C. thermocellum ethanol pathway. The red colored AdhE (Cthe_0423) is already expressed and tested in C. bescii[26]. b The T. pseudethanolicus 39E ethanol pathway. The green colored AdhE (Teth39_0206) and blue colored AdhB (Teth39_0218) are expressed and tested in C. bescii in this study

【授权许可】

2015 Chung et al.

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Biotechnology for Biofuels
Cellulosic ethanol production via consolidated bioprocessing at 75 °C by engineered Caldicellulosiruptor bescii

Daehwan Chung² Minseok Cha² Elise N. Snyder² James G. Elkins¹ Adam M. Guss¹ Janet Westpheling²
[1] Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA
[2] The BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, USA
关键词: Thermoanaerobacter pseudethanolicus 39E; Alcohol dehydrogenase; Caldicellulosiruptor bescii; Metabolic engineering; Cellulosic ethanol;
Others : 1230613 DOI : 10.1186/s13068-015-0346-4

received in 2015-07-23, accepted in 2015-09-21, 发布年份 2015


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【 摘 要 】

Background

【 授权许可】

【 参考文献 】

【摘要】

【授权许可】

【参考文献】