期刊论文详细信息
Biotechnology for Biofuels
Analysis of pectin mutants and natural accessions of Arabidopsis highlights the impact of de-methyl-esterified homogalacturonan on tissue saccharification
Fedra Francocci1  Elisa Bastianelli1  Vincenzo Lionetti1  Simone Ferrari1  Giulia De Lorenzo1  Daniela Bellincampi1  Felice Cervone1 
[1] Dipartimento di Biologia e Biotecnologie “Charles Darwin”, Sapienza Università di Roma, Piazzale Aldo Moro 5, Rome 00185, Italy
关键词: Arabidopsis thaliana;    Homogalacturonan;    Pectin;    Plant cell wall;    Saccharification;   
Others  :  794655
DOI  :  10.1186/1754-6834-6-163
 received in 2013-07-31, accepted in 2013-10-25,  发布年份 2013
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【 摘 要 】

Background

Plant biomass is a potentially important renewable source of energy and industrial products. The natural recalcitrance of the cell walls to enzymatic degradation (saccharification), which plants have evolved to defend themselves from biotic stresses, represents a major bottleneck for the industrial bioconversion of lignocellulosic biomasses. The identification of factors that influence the cell wall recalcitrance to saccharification may help to overcome the existing limitations that hamper the utilization of biomass.

Results

Here we have investigated in Arabidopsis thaliana the impact of homogalacturonan (HG) content and structure on tissue saccharification. We characterized mutants affected in genes encoding proteins involved in HG biosynthesis (quasimodo2-1; qua2-1) and methylesterification (pectin methylesterase 3; pme3). We also analyzed the natural variation of Arabidopsis through the characterization of a nested core collection of 24 accessions generated to maximize genetic variability. We found a negative correlation between the level of de-methyl-esterified HG (HGA) and cellulose degradability.

Conclusions

We propose to use the level of HGA domains as a biochemical marker of the cell wall recalcitrance to saccharification. This may be utilized for selecting, on a large scale, natural variants or mutants with improved bioconversion features.

【 授权许可】

   
2013 Francocci et al.; licensee BioMed Central Ltd.

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