A visible, targeted high-efficiency gene delivery and transfection strategy

BMC Biotechnology

Qiao-Ying Yuan²  Jing Huang³  Bao-Cheng Chu¹  Xing-Sheng Li³  Liang-Yi Si² 

[1] BioMolecular Imaging Center, Department of Radiology, University of Washington, Seattle, WA 98109, USA

[2] Department of Geriatrics, Southwest Hospital, the Third Military Medical University, Chongqing 400038, China

[3] Institute of Ultrasound Imaging, The Chongqing University of Medical Sciences, Chongqing 400010, China

关键词: angiogenesis;    gene expression;    microbubbles;    angiogenic gene;    Intramyocardial delivery;   

Others  :  1146186
DOI  :  10.1186/1472-6750-11-56

 received in 2010-12-11, accepted in 2011-05-21,  发布年份 2011

【 摘 要 】

Background

To enhance myocardial angiogenic gene expression, a novel gene delivery strategy was tested. Direct intramyocardial injection of an angiogenic gene with microbubbles and insonation were applied in a dog animal model. Dogs received one of the four different treatments in conjunction with either the enhanced green fluorescence protein (EGFP) gene or the hepatocyte growth factor (HGF) gene: gene with microbubbles (MB) and ultrasound (US); gene with US; gene with MB; or the gene alone.

Results

Distribution of MB and the gene in the myocardium was visualized during the experiment. Compared with the EGFP gene group, an average 14.7-fold enhancement in gene expression was achieved in the EGFP+MB/US group (P < 0.01). Compared with the HGF gene group, an average 10.7-fold enhancement in gene expression was achieved in the HGF+MB/US group (P < 0.01). In addition, capillary density increased from 20.8 ± 3.4/mm2 in the HGF gene group to 146.7 ± 31.4/mm2 in HGF+MB/US group (P < 0.01).

Conclusions

Thus, direct intramyocardial injection of an angiogenic gene in conjunction with microbubbles plus insonation synergistically enhances angiogenesis. This method offers an observable gene delivery procedure with enhanced expression efficiency of the delivered gene.

【 授权许可】

   
2011 Yuan et al; licensee BioMed Central Ltd.

【 图 表 】

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