A visible, targeted high-efficiency gene delivery and transfection strategy

BMC Biotechnology

Research Article

Bao-Cheng Chu¹  Liang-Yi Si²  Qiao-Ying Yuan²  Jing Huang³  Xing-Sheng Li³ 

[1] BioMolecular Imaging Center, Department of Radiology, University of Washington, 98109, Seattle, WA, USA;Department of Geriatrics, Southwest Hospital, the Third Military Medical University, 400038, Chongqing, China;Institute of Ultrasound Imaging, The Chongqing University of Medical Sciences, 400010, Chongqing, China;

关键词: Intramyocardial delivery;    angiogenic gene;    microbubbles;    gene expression;    angiogenesis;   

DOI  :  10.1186/1472-6750-11-56

 received in 2010-12-11, accepted in 2011-05-21,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundTo enhance myocardial angiogenic gene expression, a novel gene delivery strategy was tested. Direct intramyocardial injection of an angiogenic gene with microbubbles and insonation were applied in a dog animal model. Dogs received one of the four different treatments in conjunction with either the enhanced green fluorescence protein (EGFP) gene or the hepatocyte growth factor (HGF) gene: gene with microbubbles (MB) and ultrasound (US); gene with US; gene with MB; or the gene alone.ResultsDistribution of MB and the gene in the myocardium was visualized during the experiment. Compared with the EGFP gene group, an average 14.7-fold enhancement in gene expression was achieved in the EGFP+MB/US group (P < 0.01). Compared with the HGF gene group, an average 10.7-fold enhancement in gene expression was achieved in the HGF+MB/US group (P < 0.01). In addition, capillary density increased from 20.8 ± 3.4/mm2 in the HGF gene group to 146.7 ± 31.4/mm2 in HGF+MB/US group (P < 0.01).ConclusionsThus, direct intramyocardial injection of an angiogenic gene in conjunction with microbubbles plus insonation synergistically enhances angiogenesis. This method offers an observable gene delivery procedure with enhanced expression efficiency of the delivered gene.

【 授权许可】

Unknown   
© Yuan et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.