Reproduction in porcine circovirus type 2 (PCV2) seropositive gilts inseminated with PCV2b spiked semen

Acta Veterinaria Scandinavica

Giuseppe Sarli²  Federico Morandi²  Serena Panarese²  Barbara Bacci²  Domenico Ferrara²  Carlo Bianco²  Laura Fusaro²  Maria Laura Bacci²  Giovanna Galeati²  Michele Dottori⁶  Paolo Bonilauri⁶  Davide Lelli⁴  Giorgio Leotti³  Thais Vila¹  Francois Joisel¹  Gordon Allan⁵  Cinzia Benazzi²  Fabio Ostanello² 

[1] Merial SAS, 29 av Tony Garnier, 69007, Lyon, France

[2] Department of Veterinary Medical Science, University of Bologna, Via Tolara di Sopra 50, 40064, Ozzano Emilia Bologna, Italy

[3] Merial Italia Spa, Strada 6, Palazzo E/5, 20090 Milanofiori, Assago, Milan, Italy

[4] Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna (IZSLER) –Section of Brescia, Via A. Bianchi 9, 25124, Brescia, Italy

[5] Queens University, Belfast, UK

[6] Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia-Romagna (IZSLER) – Section of Reggio Emilia, Via Pitagora 2, 42100, Reggio Emilia, Italy

关键词: Infection;    Semen;    Porcine circovirus type 2;    Swine;   

Others  :  789449
DOI  :  10.1186/1751-0147-54-51

 received in 2012-04-10, accepted in 2012-08-08,  发布年份 2012

【 摘 要 】

Background

Since 1999, field evidence of transplacental infection by porcine circovirus type 2 (PCV2) and reproductive failure has been reported in pigs. The objective of this study was to evaluate the clinical and pathological consequences of PCV2 infection in conventional PCV2-seropositive gilts by insemination with PCV2b-spiked semen.

Results

Six PCV2 seropositive gilts were inseminated with PCV2b-supplemented semen (infected) and three animals with semen and cell culture medium (controls). Only three out of the six infected animals were pregnant by ultrasonography on day 29 after insemination, while two out of the three controls were pregnant. One control gilt aborted on day 23 after insemination but not due to PVC2. Viraemia was demonstrated in four out of six infected and in one control gilt that became infected with PCV2a. Anti-PCV2 antibody titres showed dynamic variations in the infected group throughout the study. Among infected gilts, the animal with the lowest anti-PCV2 titre (1/100) at the beginning of the experiment and another that reached a similar low value during the experiment showed evident seroconversion over time and had also PCV2 positive foetuses. One placenta displayed mild focal necrosis of the chorionic epithelium positively stained by immunohistochemistry for PCV2 antigen.

Conclusions

PCV2-seropositive gilts can be infected with PCV2 after intrauterine exposure and low maternal antibody titre may increase the probability of a foetal infection.

【 授权许可】

   
2012 Sarli et al.; licensee BioMed Central Ltd.

【 图 表 】

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