Bacteriophage-encoded lytic enzymes control growth of contaminating Lactobacillus found in fuel ethanol fermentations

Biotechnology for Biofuels

David M Donovan²  Stephen R Hughes¹  Kenneth M Bischoff¹  Piyum A Khatibi¹  Dwayne R Roach² 

[1]US Department of Agriculture, National Center for Agricultural Utilization Research, Agricultural Research Service, Peoria, IL, 61604, USA

[2]US Department of Agriculture, Animal Biosciences and Biotechnology Laboratory, Animal & Natural Resources Institute, Agricultural Research Service, Beltsville, MD 20705, USA

关键词: Lactobacilli;    Lactobacillus;    Lactic acid bacteria;    Contamination;    Fermentation;    Ethanol;    Peptidoglycan;    endolysin;    Lysin;    Bacteriophage;   

Others  :  798155
DOI  :  10.1186/1754-6834-6-20

 received in 2012-09-21, accepted in 2013-01-11,  发布年份 2013

【 摘 要 】

Background

Reduced yields of ethanol due to bacterial contamination in fermentation cultures weaken the economics of biofuel production. Lactic acid bacteria are considered the most problematic, and surveys of commercial fuel ethanol facilities have found that species of Lactobacillus are predominant. Bacteriophage lytic enzymes are peptidoglycan hydrolases that can degrade the Gram positive cell wall when exposed externally and provide a novel source of antimicrobials that are highly refractory to resistance development.

Results

The streptococcal phage LambdaSa2 (λSa2) endolysin demonstrated strong lytic activity towards 17 of 22 strains of lactobacilli, staphylococci or streptococci and maintained an optimal specific activity at pH 5.5 and in the presence of ≤ 5% ethanol (fermentation conditions) toward L. fermentum. Lactobacillus bacteriophage endolysins LysA, LysA2 and LysgaY showed exolytic activity towards 60% of the lactobacilli tested including four L. fermentum isolates from fuel ethanol fermentations. In turbidity reduction assays LysA was able to reduce optical density >75% for 50% of the sensitive strains and >50% for the remaining strains. LysA2 and LysgaY were only able to decrease cellular turbidity by <50%. Optimal specific activities were achieved for LysA, LysA2, and LysgaY at pH 5.5. The presence of ethanol (≤5%) did not reduce the lytic activity. Lysins were able to reduce both L. fermentum (BR0315-1) (λSa2 endolysin) and L. reuteri (B-14171) (LysA) contaminants in mock fermentations of corn fiber hydrolysates.

Conclusion

Bacteriophage lytic enzymes are strong candidates for application as antimicrobials to control lactic acid bacterial contamination in fuel ethanol fermentations.

【 授权许可】

   
2013 Roach et al.; licensee BioMed Central Ltd.

【 图 表 】

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