Antibiotic resistance in potential probiotic lactic acid bacteria of fermented foods and human origin from Nigeria

BMC Microbiology

Research

Manvesh Kumar Sihag¹  Arundhati Ganesh Wandhare²  Harsh Panwar²  Anamika Singh²  Vikas Sangwan²  Rachael T. Duche³  Tochukwu N. T. Nwagu⁴  Lewis. I. Ezeogu⁵ 

[1] Department of Dairy Chemistry, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India;Department of Dairy Microbiology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India;Department of Dairy Microbiology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India;Department of Microbiology, Federal University of Agriculture Makurdi-Nigeria, Makurdi, Nigeria;Department of Microbiology, University of Nigeria Nsukka, Nsukka, Nigeria;UNESCO International Centre for Biotechnology, Nsukka, Nigeria;Department of Microbiology, University of Nigeria Nsukka, Nsukka, Nigeria;Department of Microbiology, University of Nigeria Nsukka, Nsukka, Nigeria;UNESCO International Centre for Biotechnology, Nsukka, Nigeria;

关键词: Antibiotic resistance;    Lactic acid bacteria;    Lactobacilli;    Multi drug resistant;    Resistance genes;   

DOI  :  10.1186/s12866-023-02883-0

 received in 2022-11-17, accepted in 2023-05-06,  发布年份 2023

来源: Springer

【 摘 要 】

IntroductionProbiotic lactobacilli are generally recognized as safe (GRAS) and are being used in several food and pharma formulations. However, growing concern of antibiotic resistance in bacterial strains of food origin and its possible transmission via functional foods is increasingly being emphasized.ObjectivesThis study screened potential probiotic lactic acid bacteria (LAB) strains for their phenotypic and genotypic antibiotic resistance profiles.MethodsSusceptibility to different antibiotics was assayed by the Kirby Bauer standard disc diffusion protocol. Both conventional and SYBR-RTq-PCR were used for detection of resistance coding genes.ResultsA variable susceptibility pattern was documented against different antibiotic classes. LAB strains irrespective of origin displayed marked phenotypic resistance against cephalosporins, aminoglycosides, quinolones, glycopeptides; and methicillin among beta-lactams with few exceptions. In contrast, high sensitivity was recorded against macrolides, sulphonamides and carbapenems sub-group of beta-lactams with some variations. parC, associated with ciprofloxacin resistance was detected in 76.5% of the strains. Other prevalent resistant determinants observed were aac(6?)Ii (42.1%), ermB, ermC (29.4%), and tetM (20.5%). Six (?17.6%) of the isolates were free from genetic resistance determinants screened in this study.ConclusionStudy revealed presence of antibiotic resistance determinants among lactobacilli from both fermented foods and human sources.

【 授权许可】

CC BY   
© The Author(s) 2023

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