Methicillin-resistant Staphylococcus aureus containing mecC in Swedish dairy cows

Acta Veterinaria Scandinavica

Helle Ericsson Unnerstad¹  Björn Bengtsson¹  Margareta Horn af Rantzien¹  Stefan Börjesson¹ 

[1] Department of Animal Health and Antimicrobial Strategies, National Veterinary Institute, Uppsala, SE-751 89, Sweden

关键词: Dairy cows;    Mastitis;    mecC;    Staphylococcus aureus;    MRSA;   

Others  :  789417
DOI  :  10.1186/1751-0147-55-6

 received in 2012-11-02, accepted in 2013-01-28,  发布年份 2013

【 摘 要 】

Background

Hitherto, methicillin-resistant Staphylococcus aureus (MRSA) has not been detected in Swedish cattle. However, due to the report of mecC, a novel homologue to the mecA gene, there was reason to re-evaluate susceptibility results from strain collections of Staphylococcus aureus and test suspected isolates for the presence of mecC.

Findings

Bovine isolates of S. aureus with elevated minimum inhibitory concentrations of beta-lactams were retrospectively tested for presence of mecC. In four of the isolates mecC was detected.

Conclusion

In Sweden, this is the first finding of MRSA in cattle and the first detection of MRSA harbouring mecC of domestic animal origin. MRSA in animal populations has implications as a potential reservoir with risk for spread to humans. Occurrence of MRSA among Swedish cattle appears still very limited.

【 授权许可】

   
2013 Unnerstad et al.; licensee BioMed Central Ltd.

【 参考文献 】

• [1]Deurenberg RH, Stobberingh EE: The evolution of Staphylococcus aureus. Infec Genet Evol 2008, 8:747-763.
• [2]Weese JS, Van Duijkeren E: Methicillin-resistant Staphylococcus aureus and Staphylococcus pseudintermedius in veterinary medicine. Vet Microbiol 2010, 140:418-429.
• [3]van der Wolf PJ, Rothkamp A, Junker K, De Neeling AJ: Staphylococcus aureus (MSSA) and MRSA (CC398) isolated from post-mortem samples from pigs. Vet Microbiol 2012, 158:136-141.
• [4]Vanderhaeghen W, Cerpentier T, Adriaensen C, Vicca J, Hermans K, Butaye P: Methicillin-resistant Staphylococcus aureus (MRSA) ST398 associated with clinical and subclinical mastitis in Belgian cows. Vet Microbiol 2010, 144:166-171.
• [5]European Food Safety Authority: Scientific opinion of the panel on biological hazards on a request from the European Commission on assessment of the public health significance of meticillin resistant Staphylococcus aureus (MRSA) in animals and foods. The EFSA Journal. 2009, 993:1-73.
• [6]Garcia-Alvarez L, Holden TG, Lindsay H, Webb CR, Brown DFJ, Curran MD, Walpole E, Brooks K, Pickard DJ, Teale C, Parkhill J, Bentley SD, Edwards GF, Girvan EK, Kaerns AM, Pichon B, Hill RLR, Larsen AR, Skov RL, Peacock SJ, Maskell DJ, Holmes MA: Meticillin-resistant Staphylococcus aureus with a novel mecA homologue in human and bovine populations in the UK and Denmark: a descriptive study. Lancet Infect Dis 2011, 11:595-603.
• [7]Shore AC, Deasy EC, Slickers P, Brennan G, O’Connell B, Monecke S, Ehricht R, Coleman DC: Detection of staphylococcal cassette chromosome mec type XI carrying highly divergent mecA, mecI, mecR1, blaZ, and ccr genes in human clinical isolates of clonal complex 130 methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 2011, 55:3765-3773.
• [8]European Center for Disease Prevention and Control: Antimicrobial resistance surveillance in Europe 2010. Sweden: ECDC, Stockholm; 2011. http://www.ecdc.europa.eu/en/publications/Publications/1111_SUR_AMR_data.pdf.pdf webcite
• [9]Ericsson Unnerstad H, Lindberg M, Börjesson S, Grönlund Andersson U: Screening for methicillin-resistant Staphylococcus aureus (MRSA) in pigs and horses in Sweden [abstract]. In Proceedings of the 2nd ASM-ESCMID Conference on Methicillin-resistant Staphylococci in Animals: Veterinary and Public Health Implications: 8–11 September, 2011. Washington, DC; 2011:47.
• [10]Bryan LE, Godfrey AJ: Beta-lactam antibiotics: mode of action and bacterial resistance. In Antibiotics in laboratory medicine. Edited by Baltimore LV. USA: Williams & Wilkins; 1991:648.
• [11]Clinical and Laboratory Standards Institute: Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals; approved standard. 3rd edition. Wayne, PA: M31-A3. CLSI; 2008.
• [12]Harmsen D, Claus H, Witte W, Rothganger J, Turnwald D, Vogel U: Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management. J Clin Microbiol 2003, 41:5442-5448.
• [13]Enright MC, Day NP, Davies CE, Peacock SJ, Spratt BG: Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J Clin Microbiol 2000, 38:1008-1015.
• [14]Murchan SM, Kaufmann E, Deplano A, De Ryck R, Struelens M, Zinn CE, Fussing V, Salmenlinna S, Vuopio-Varkila J, El Solh N, Cuny C, Witte W, Tassios PT, Legakis N, Leeuwen W, Van Belkum A, Vindel A, Laconcha I, Garaizar J, Haeggman S, Olsson-Liljequist B, Ransjo U, Coombes G, Cookson B: Harmonization of pulsed-field gel electrophoresis protocols for epidemiological typing of strains of methicillin-resistant Staphylococcus aureus: a single approach developed by consensus in 10 European laboratories and its application for tracing the spread of related strains. J Clin Microbiol 2003, 41:1574-1585.
• [15]Francois P, Renzi G, Pittet D, Bento M, Lew D, Harbarth S, Vaudaux P, Schrenzel J: A novel multiplex real-time PCR assay for rapid typing of major staphylococcal cassette chromosome mec elements. J Clin Microbiol 2004, 42:3309-3312.
• [16]Swedish Institute for Communicable Disease Control: SWEDRES 2011. A report on Swedish antibiotic utilisation and resistance in human medicine. 2012. http://www.smittskyddsinstitutet.se/upload/Publikationer/SWEDRES-SVARM-2011_2012-15-3.pdf webcite
• [17]Cuny C, Layer F, Strommenger B, Witte W: Rare occurrence of methicillin-resistant Staphylococcus aureus CC130 with a novel mecA homologue in humans in Germany. PLoS One 2011, 6:e24360.
• [18]Kriegeskorte A, Ballhausen B, Idelevich EA, Kock R, Friedrich AW, Karch H, Peters G, Becker K: Human MRSA isolates with novel genetic homolog, Germany. Emerg Infect Dis 2012, 18:1016-1018.