Occurrence and characterization of methicillin-resistant staphylococci from bovine mastitis milk samples in Finland

Acta Veterinaria Scandinavica

Veera Gindonis⁵  Suvi Taponen⁵  Anna-Liisa Myllyniemi⁴  Satu Pyörälä¹  Suvi Nykäsenoja⁴  Saara Salmenlinna³  Laura Lindholm²  Merja Rantala⁶ 

[1] Faculty of Veterinary Medicine, Department of Production Animal Medicine, University of Helsinki, Paroninkuja 20, 04920 Saarentaus, Finland

[2] Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare, PO Box 57, 20521 Turku, Finland

[3] Department of Infectious Disease Surveillance and Control, National Institute for Health and Welfare, PO Box 30, 00271 Helsinki, Finland

[4] Research and Laboratory Department, Food and Feed Microbiology Research Unit, Finnish Food Safety Authority Evira, Mustialankatu 3, 00790 Helsinki, Finland

[5] Faculty of Veterinary Medicine, Department of Production Animal Medicine, University of Helsinki, Koetilantie 7, 00014 Helsinki, Finland

[6] Faculty of Veterinary Medicine, Department of Equine and Small Animal Sciences, University of Helsinki, Viikintie 49, 00014 Helsinki, Finland

关键词: mecC;    mecA;    Mastitis;    Bovine;    SCCmec;    Methicillin resistance;    Staphylococci;   

Others  :  789273
DOI  :  10.1186/1751-0147-55-61

 received in 2013-02-13, accepted in 2013-08-19,  发布年份 2013

【 摘 要 】

Background

Methicillin-resistant staphylococci (MRS) are increasingly being isolated in bovine mastitis. The aim of our study was to evaluate the occurrence of MRS in Finnish mastitis milk samples and characterize the MRS isolates using molecular methods.

Results

Methicillin-resistant S. aureus (MRSA) was a rare finding in bovine mastitis in Finland. Only two out of 135 (1.5%) S. aureus isolates were positive for mec genes. One of these carried mecA and was of spa type t172, SCCmec type IV and ST375, and the other harboured mecC, being spa type t3256, and ST130. MRSA ST375 is common among human MRSA isolates in Finland, but this is the first report in the country of bovine mecC MRSA. In coagulase-negative staphylococci (CoNS) originating from bovine mastitis, methicillin resistance was more common. In the two CoNS collections studied, 5.2% (17/324) and 1.8% (2/110) of the isolates were mecA positive. Eighteen of these were methicillin-resistant S. epidermidis (MRSE), which were divided into 6 separate PFGE clusters. One pulsotype was detected in different parts of the country, indicating clonal spread. Most MRSE (13/18) were of SCCmec type IV, one was of type V and four were non-typeable. Comparison with a human staphylococcal database indicated that bovine MRSE strains were not closely related to human MRSE isolates.

Conclusions

The occurrence of MRS, especially MRSA, in bovine mastitis in Finland was low. Most methicillin-resistant bovine CoNS are MRSE, and we found evidence of a bovine MRSE strain that may spread clonally. This is the first report of a Finnish bovine isolate of MRSA_mecC ST130. The study provides a baseline for further MRS monitoring.

【 授权许可】

   
2013 Gindonis et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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