Sorghum mutant RG displays antithetic leaf shoot lignin accumulation resulting in improved stem saccharification properties

Biotechnology for Biofuels

Carloalberto Petti¹  Anne E Harman-Ware²  Mizuki Tateno¹  Rekha Kushwaha¹  Andrew Shearer¹  A Bruce Downie¹  Mark Crocker²  Seth DeBolt¹ 

[1] Plant Physiology, Department of Horticulture, Agricultural Science Center North, University of Kentucky, Lexington, KY 40546, USA

[2] Center for Applied Energy Research, University of Kentucky, 2540 Research Park Drive, Lexington, KY 40511, USA

关键词: Lignin;    Biofuel;    Lignocellulose;    Cell wall;    Sorghum;    Phenylpropanoid;   

Others  :  797845
DOI  :  10.1186/1754-6834-6-146

 received in 2013-06-10, accepted in 2013-09-24,  发布年份 2013

【 摘 要 】

Background

Improving saccharification efficiency in bioenergy crop species remains an important challenge. Here, we report the characterization of a Sorghum (Sorghum bicolor L.) mutant, named REDforGREEN (RG), as a bioenergy feedstock.

Results

It was found that RG displayed increased accumulation of lignin in leaves and depletion in the stems, antithetic to the trend observed in wild type. Consistent with these measurements, the RG leaf tissue displayed reduced saccharification efficiency whereas the stem saccharification efficiency increased relative to wild type. Reduced lignin was linked to improved saccharification in RG stems, but a chemical shift to greater S:G ratios in RG stem lignin was also observed. Similarities in cellulose content and structure by XRD-analysis support the correlation between increased saccharification properties and reduced lignin instead of changes in the cellulose composition and/or structure.

Conclusion

Antithetic lignin accumulation was observed in the RG mutant leaf-and stem-tissue, which resulted in greater saccharification efficiency in the RG stem and differential thermochemical product yield in high lignin leaves. Thus, the red leaf coloration of the RG mutant represents a potential marker for improved conversion of stem cellulose to fermentable sugars in the C4 grass Sorghum.

【 授权许可】

   
2013 Petti et al.; licensee BioMed Central Ltd.

【 图 表 】

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