First detection of Klebsiella variicola producing OXA-181 carbapenemase in fresh vegetable imported from Asia to Switzerland

Antimicrobial Resistance and Infection Control

R. Stephan³  J. Klumpp²  P. Nordmann¹  L. Poirel¹  K. Zurfluh³ 

[1]Department of Medicine, Faculty of Science, Medical and Molecular Microbiology Unit “Emerging Antibiotic Resistance”, University of Fribourg, Fribourg, Switzerland

[2]Institute of Food, Nutrition and Health, ETH Zürich, Schmelzbergstrasse 7, Zürich, 8092, Switzerland

[3]Institute for Food Safety and Hygiene, Vetsuisse Faculty University of Zurich, Winterthurerstrasse 272, Zurich, 8057, Switzerland

关键词: OXA-181;    IncX3 plasmid;    Enterobacteriaceae;    Food;    Carbapenemase;   

Others  :  1230966
DOI  :  10.1186/s13756-015-0080-5

 received in 2015-06-17, accepted in 2015-09-28,  发布年份 2015

【 摘 要 】

Background

The emergence and worldwide spread of carbapenemase-producing Enterobacteriaceae is of great concern to public health services. The aim of this study was to investigate the occurrence of carbapenemase-producing Enterobacteriaceae in fresh vegetables and spices imported from Asia to Switzerland.

Findings

Twenty-two different fresh vegetable samples were purchased in March 2015 from different retail shops specializing in Asian food. The vegetables included basil leaves, bergamont leaves, coriander, curry leaves, eggplant and okra (marrow). Samples had been imported from Thailand, the Socialist Republic of Vietnam and India. After an initial enrichment-step, carbapenemase-producing Enterobacteriaceae were isolated from two carbapenem-containing selective media (SUPERCARBA II and Brilliance CRE Agar). Isolates were screened by PCR for the presence of bla_KPC , bla_NDM , bla_OXA-48-likeand bla_VIM . An OXA-181-producing Klebsiella variicola was isolated in a coriander sample with origin Thailand/Vietnam. The bla_OXA-181gene was encoded in a 14′027 bp region flanked by two IS26-like elements on a 51-kb IncX3-type plasmid.

Conclusions

The results of this study suggest that the international production and trade of fresh vegetables constitute a possible route for the spread of carbapenemase-producing Enterobacteriaceae. The presence of carbapenemase-producing organisms in the food supply is alarming and an important food safety issue.

【 授权许可】

   
2015 Zurfluh et al.

【 参考文献 】

• [1]Zurfluh K, Nüesch-Inderbinen M, Poirel L, Nordmann P, Hächler H, Stephan R. Emergence of Escherichia coli producing OXA-48 beta-lactamase in the community in Switzerland. Antimicrob Res Infect Control. 2015; 4:9. BioMed Central Full Text
• [2]Cantón R, Akóva M, Carmeli Y, Giske CG, Glupczynski Y, Gniadowski M, Livermore DM, Miriagou V, Naas T, Rossolini GM, Samuelsen O, Seifert H, Woodford N, Nordmann P. Rapid evolution and spread of carbapenemases among Enterobacteriaceae in Europe. Clin Microbiol Infect. 2012; 18:413-31.
• [3]Zurfluh K, Nüesch-Inderbinen M, Morach M, Zihler Berner A, Hächler H, Stephan R. Extended-spectrum ß-lactamase-producing-Enterobacteriaceae in vegetables imported from the Dominican Republic, India, Thailand and Vietnam. Appl Environ Microbiol. 2015; 81:3115-20.
• [4]Ellington MJ, Kistler J, Livermore DM, Woodford N. Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases. J Antimicrob Chemother. 2007; 59:321-2.
• [5]Poirel L, Walsh TR, Cuvillier V, Nordmann P. Multiplex PCR for detection of acquired carbapenemase genes. Diagn Microbiol Infect Dis. 2011; 70:119-23.
• [6]Zurfluh K, Jakobi G, Stephan R, Hächler H, Nüesch-Inderbinen M. Replicon typing of plasmids carrying blaCTX-M-1 in Enterobacteriaceae of animal, environmental and human origin. Front Microbiol. 2014; 5:555.
• [7]He S, Burgess Hickman A, Varani AM, Siguier P, Chandler M, Dekker JP, Dydaa F. Insertion sequence IS26 reorganizes plasmids in clinically isolated multidrug-resistant bacteria by replicative transposition. mBio. 2015; 6(3):e00762-15.
• [8]Performance Standards for Antimicrobial Susceptibility Testing. Twenty-First Informational Supplement. CLSI document M100–S23. Clinical and Laboratory Standarts Institute, Wayne; 2013.
• [9]Evans BA, Amyes SGB. OXA β-lactamases. Clin Microbiol Rev. 2014; 27:241-63.
• [10]Nordmann P, Poirel L. The difficult-to-control spread of carbapenemase producers among Enterobacteriaceae worldwide. Clin Microbiol Infect. 2014; 20:821-30.
• [11]Castanheira M, Deshpande LM, Mathai D, Bell JM, Jones RN, Mendes RE. Early dissemination of NDM-1- and OXA-181-producing Enterobacteriaceae in Indian hospitals: report from the SENTRY Antimicrobial Surveillance Program, 2006-2007. Antimicrob Agents Chemother. 2011; 55:1274-8.
• [12]Potron A, Poirel L, Nordmann P. Origin of OXA-181, an emerging carbapenem-hydrolyzing oxacillinase, as a chromosomal gene in Shewanella xiamenensis. Antimicrob Agents Chemother. 2011; 55:4405-7.
• [13]Villa L, Carattoli A, Nordmann P, Carta C, Poirel L. Complete sequence of the IncT-type plasmid pT-OXA-181 carrying the blaOXA-181 carbapenemase gene from Citrobacter freundii. Antimicrob Agents Chemother. 2013; 57:1965-7.
• [14]Liu Y, Yu F, Wu W, Xie Y, Wang X, Zhang X, Chen X, Zong Z. OXA-181-producing Escherichia coli in China: the first report and characterization using whole genome sequencing. Antimicrob Agents Chemother. in press.
• [15]Veldman K, Kant A, Dierikx C, van Essen-Zandbergen A, Wit B, Mevius D. Enterobacteriaceae resistant to third-generation cephalosporins and quinolones in fresh culinary herbs imported from Southeast Asia. Int J Food Microbiol. 2014; 177:72-7.