Association of decreased mitochondrial DNA content with the progression of colorectal cancer

BMC Cancer

XiaoMing Guo²  Ying Han³  XiaoPeng Wang¹  Wei Xu¹  ZhenHua Zhang¹  YunXin Zhang¹  ZhiJing Wang¹  Ping Huang¹  HaiHong Cui¹ 

[1]Department of Gastroenterology, 305 Hospital of PLA, Beijing, China

[2]Department of Pathology, Institute of radiation medicine, Beijing, China

[3]Department of Gastroenterology, Beijing Military General Hospital of PLA, Beijing, China

关键词: Prognosis;    Quantitative PCR;    Copy number;    Mitochondrial DNA;    Colorectal cancer;   

Others  :  1079880
DOI  :  10.1186/1471-2407-13-110

 received in 2012-07-13, accepted in 2013-02-21,  发布年份 2013

【 摘 要 】

Background

Experimental data suggest that mitochondria is involved in tumorigenesis. However, little is known about the qualitative and quantitative changes of mtDNA in colorectal cancer tissues. We therefore conducted possible correlations of the mitochondrial DNA (mtDNA) copy number in colorectal cancer (CRC) with clinical and pathological findings and CRC prognosis.

Methods

mtDNA copy numbers in CRC cancer tissue and adjacent non-cancerous tissue samples were measured using quantitative real-time polymerase chain reaction analyses from 60 patients admitted to our hospital. We examined the correlation of mtDNA copy numbers and clinicopathologic parameters of CRC patients. The correlation between mtDNA copy number and three-year survival was analyzed.

Results

The mtDNA copy number was lower in CRC tissue compared with the corresponding non-cancerous colorectal tissue (mean: 108.60 ± 20.11 vs. 153.68 ± 25.72) and was significantly correlated with lymph-node metastasis. Patients with a lower mtDNA copy number tended to have lower 3-year survival than patients with a higher mtDNA copy number assessed by Kaplan–Meier curves, but the correlation was not significant (overall survival, 63.0 vs 83%).

Conclusions

These results suggest that a reduced copy number of mtDNA is correlated with malignant potential in CRC.

【 授权许可】

   
2013 Cui et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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