Using Barnacle-Specific Primers to Quantify Larva Attachment on Nano-Filled Coatings during the Early Stages of Marine Biofouling

2018 5th International Conference on Advanced Composite Materials and Manufacturing Engineering

Yan, Zhengyu^1 ; Guo, Changlu^1 ; Zhang, Zhizhou^1,2 ; Gao, Shan^2

School of Marine Science and Technology, Marine Anti-fouling Engineering Technology Center of Shandong Province, Harbin Institute of Technology, Harbin, China^1

Shandong Gredmedic Co., Ltd, Weihai, China^2

关键词: 18S rDNA;    Antifouling;    Biofilm dynamics;    Copy number;    Marine biofilms;    Quantitative real time PCR;    Species specifics;    Specific primers;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/394/4/042025/pdf
DOI  :  10.1088/1757-899X/394/4/042025

来源: IOP

【 摘 要 】

This study tried to set up an approach to quantify the attached barnacle larvae in marine biofilms. Biofilms were collected from nano-filled coatings that previously characterized with different antifouling scores for 3-months. Quantification was achieved by 18S rDNA copy number measurement in quantitative real-time PCR (qPCR) using barnacle-species specific PCR (polymerase chain reaction) primers. Interestingly, the coatings with best anti-fouling scores were not the ones with less barnacle larva attachment in the early stage of marine biofouling, suggesting that some nano-filled coating be able to attract barnacle larvae's attachment but can prevent their further growth either by directly inhibiting the growth or evacuating the larvae through modulated biofilm dynamics, and the relative amount of barnacle larvae attachment cannot be regarded as a prediction for long-term antifouling efficacy of a coating.