Interaction betwen Lead and Bone Protein to Affect Bone Calcium Level Using UV-Vis Spectroscopy

International Conference on Nanomaterials and Biomaterials 2017

材料科学;物理学;生物科学

Noor, Z.^1 ; Azharuddin, A.^2 ; Aflanie, I.^3 ; Kania, N.^4 ; Suhartono, E.^5

Department of Orthopaedic Surgery, Ulin General Hospital, Faculty of Medicine, Lambung Mangkurat University, South Kalimantan, Banjarmasin

70232, Indonesia^1

Department of Orthopaedic Surgery, Faculty of Medicine, Syiah Kuala University, Aceh

24415, Indonesia^2

Department of Forensic, Ulin General Hospital, Faculty of Medicine, Lambung Mangkurat University, South Kalimantan, Banjarmasin

70232, Indonesia^3

Research Center for Toxicology, Cancer and Degenerative Disease, Department of Pathology, Ulin General Hospital, Faculty of Medicine, Lambung Mangkurat University, South Kalimantan, Banjarmasin

70232, Indonesia^4

Department of Medical Chemistry/Biochemistry, Faculty of Medicine, Lambung Mangkurat University, Banjarbaru, South Kalimantan

70712, Indonesia^5

关键词: Binding constant;    Bone calcium;    Control groups;    High affinity;    Male rats;    Spectro-photometric method;    Study design;    UV-vis spectroscopy;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/350/1/012009/pdf
DOI  :  10.1088/1757-899X/350/1/012009

来源: IOP

【 摘 要 】

This present study aim to evaluate the interactions between lead (Pb) and with bone protein by UV-Vis approach. In addition, this prsent study also aim to investigate the effect of Pb on bone calcium (Ca) level. The present study was a true experimental study design to examine the impact of Pb exposure in bone of male rats (Rattus novergicus). The study involved 5 groups, P1 was the control group, while the other (P2-P5) were the case group with exposure of Pb in different concentration within 4 weeks. At the end of the exposure, the interaction between Pb and protein was determined using UV-Vis spectrophotometric method, and the Ca level was determined using permanganometric method. The results shows that that there is an interaction between Pb and bone protein. The result also shows that the value of the binding constant of Protein-Pb is 32.71. It means Pb have an high affinity to bind with bone protein, which promote a further reaction to induced the release of bone Ca from the bone protein. In conclusion, this present study found an obvious relationship between Pb and bone protein which promote a further reaction to increase the releasing of bone calcium.