The Acute Toxicity Test of Methanolic Extract of Hyptis pectinata Poit on Liver Balb/c Mice

11th Joint Conference on Chemistry in Conjunction with the 4th Regional Biomaterials Scientific Meeting

材料科学;化学;生物科学

Suzery, M.^1 ; Cahyono, B.^1 ; Astuti, P.^2

Department of Chemistry, Faculty of Science and Mathematics, Diponegoro University, Semarang, Indonesia^1

Physiology and Microanatomy Division, Faculty of Veterinary Medicine, Gadjah Mada University, Jogjakarta, Indonesia^2

关键词: Acute toxicity test;    Control groups;    Harmful effects;    Kruskal-Wallis tests;    Lamiaceae family;    Methanolic extracts;    Physiological activity;    Treatment group;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/172/1/012029/pdf
DOI  :  10.1088/1757-899X/172/1/012029

来源: IOP

【 摘 要 】

Plants from Lamiaceae family has almost entirely reported having physiological activities. One of them is Hyptis pectinata Poit plant. Research on the toxicity of Hyptis pectinata needs to be done to protect people from the possibility of its harmful effects. This study aim to know the acute toxicity effects of Hyptis pectinata extract (HPE) on liver of Balb/c mice. This research was a laboratory experimental study using the post test only controlled group design. Balb/c mice were randomly divided into 4 groups. K (control group), P1, P2 and P3 (treatment groups; given HPE 200mg/kgBW, 1000 mg/kgBW, and 5000 mg/kgBW, respectively). The extract was orally given with gastric tube on the first day and the mice were terminated at the 8th day then the livers were observed. The Kruskal-Wallis test for macroscopic morphological and volume of the liver showed there were no significant difference with p=0.406 and p=0.054. The highest liver histopathological score was in P3 group. The Kruskal-Wallis test showed significantly difference (p=0.000). Continued with Mann-Withney test that showed a significant difference in K-P1 (p=0.009), K-P2 (p=0.009), K-P3 (p=0.009), P1-P2 (p=0.028), and P1-P3 (p=0.009). In conclusion, the HPE is safe to use which has no complication with liver of mice.