Experimental Study on 3D Chi - Hap Scaffolds for Thyroid Cartilage Repairing

5th Annual International Conference on Material Science and Environmental Engineering

材料科学;生态环境科学

Sun, Nannan^1 ; Shi, Tingchun^1 ; Fan, Yuan^1 ; Hu, Binbin^1

Bio-Manufacturing Research Center, Hangzhou Dianzi University, Hangzhou, China^1

关键词: Animal experiments;    Composite scaffolds;    Experimental groups;    Hematoxylin eosin;    Hydroxyapatite (HAp);    Interconnected structures;    Low-temperature deposition manufacturing;    New Zealand rabbits;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/301/1/012018/pdf
DOI  :  10.1088/1757-899X/301/1/012018

来源: IOP

【 摘 要 】

Due to the limitation of self-repairing capability for cartilage injury, the construction of tissue engineering in vitro has been an ideal treatment to repair tissue injury. In this paper, hydroxyapatite (Hap) and chitosan (Chi) were selected to fabricate the scaffold through low temperature deposition manufacturing (LDM) technique. The scaffold was characterized with interconnected structure and high porosity, as well as lower toxicity to cells (TDC-5-EGPE). Animal experiment was performed, Twelve white New Zealand rabbits were randomly divided into two groups, the side of the thyroid cartilage was removed, Chi-HAP composite scaffold was implanted into the cartilage defect as the experimental group A. Group B was treated for thyroid cartilage defects without any treatment. After 10 weeks, hematoxylin-eosin (HE) staining and S-O staining were carried out on the injured tissues. The result showed that newborn chondrocytes were found in repaired areas for group A, and there are no new cells found for group B. Therefore, Chi-HAP composite scaffolds formed by LDM possess biological activity for repairing injury cartilage.