Cryogenic Engineering Conference 2017 | |
A new passive system for contamination-free long-distance cryo-transfer of biological tissues | |
材料科学;物理学 | |
Cheng, Tian^1,2,3 ; Plane, Florent^1,2 ; Sogaard Jensen, Louise Helene^1 ; Van Den Brandt, Ben^4 ; Comment, Arnaud^3,5 ; Meibom, Anders^1,2 | |
Laboratory for Biological Geochemistry, Ecole Polytechnique Fédérale de Lausanne, Lausanne | |
CH-1015, Switzerland^1 | |
Institute of Earth Sciences, University of Lausanne, Lausanne | |
CH-1015, Switzerland^2 | |
Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge | |
CB2 0RE, United Kingdom^3 | |
Paul Scherrer Institute, Villigen | |
CH-5232, Switzerland^4 | |
Institute of Physics of Biological Systems, Ecole Polytechnique Fédérale de Lausanne, Lausanne | |
CH-1015, Switzerland^5 | |
关键词: Biological tissues; Contamination-free; Correlative study; Cryo-transfer; Passive systems; Time-periods; Total transfer; Transfer systems; | |
Others : https://iopscience.iop.org/article/10.1088/1757-899X/278/1/012123/pdf DOI : 10.1088/1757-899X/278/1/012123 |
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学科分类:材料科学(综合) | |
来源: IOP | |
【 摘 要 】
Several new analytical techniques require long-distance cryogenic transfer of samples that need to be kept at stable temperatures for long time periods, but also to be additionally contamination-free. In this study we developed a passive transfer system to fulfil those requirements. With 125mL of liquid nitrogen stored, one cryo-sectioned sample was maintained around 120±1 K and a pressure of about 3x10-7mbar for at least 2 hours. With a total transfer weight of 5 Kg this system can be easily handled and carried by any transportation means so that the same sample can be used for different imaging centres located remotely permitting correlative studies.
【 预 览 】
Files | Size | Format | View |
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A new passive system for contamination-free long-distance cryo-transfer of biological tissues | 944KB | download |