A new passive system for contamination-free long-distance cryo-transfer of biological tissues

Cryogenic Engineering Conference 2017

材料科学;物理学

Cheng, Tian^1,2,3 ; Plane, Florent^1,2 ; Sogaard Jensen, Louise Helene^1 ; Van Den Brandt, Ben^4 ; Comment, Arnaud^3,5 ; Meibom, Anders^1,2

Laboratory for Biological Geochemistry, Ecole Polytechnique Fédérale de Lausanne, Lausanne

CH-1015, Switzerland^1

Institute of Earth Sciences, University of Lausanne, Lausanne

CH-1015, Switzerland^2

Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge

CB2 0RE, United Kingdom^3

Paul Scherrer Institute, Villigen

CH-5232, Switzerland^4

Institute of Physics of Biological Systems, Ecole Polytechnique Fédérale de Lausanne, Lausanne

CH-1015, Switzerland^5

关键词: Biological tissues;    Contamination-free;    Correlative study;    Cryo-transfer;    Passive systems;    Time-periods;    Total transfer;    Transfer systems;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/278/1/012123/pdf
DOI  :  10.1088/1757-899X/278/1/012123

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

Several new analytical techniques require long-distance cryogenic transfer of samples that need to be kept at stable temperatures for long time periods, but also to be additionally contamination-free. In this study we developed a passive transfer system to fulfil those requirements. With 125mL of liquid nitrogen stored, one cryo-sectioned sample was maintained around 120±1 K and a pressure of about 3x10-7mbar for at least 2 hours. With a total transfer weight of 5 Kg this system can be easily handled and carried by any transportation means so that the same sample can be used for different imaging centres located remotely permitting correlative studies.