Influence of Long-Term Fertilization on Spore Density and Colonization of Arbuscular Mycorrhizal Fungi in a Brown Soil

1st International Conference on Frontiers of Materials Synthesis and Processing

材料科学;化学

Li, Dongdong^1,2 ; Luo, Peiyu^1,2 ; Yang, Jinfeng^1,2

College of Land and Environment, Shenyang Agricultural University, Shenyang

110866, China^1

National Engineering Laboratory for Efficient Utilization of Soil, Fertilizer Resources, Liaoning

110866, China^2

关键词: Arbuscular mycorrhizal fungi;    Colonization rate;    Inorganic fertilizers;    Long-term fertilization;    Mineral nitrogen;    Mycorrhizal colonization;    Organic fertilizers;    Sucrose density gradient centrifugation;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/274/1/012022/pdf
DOI  :  10.1088/1757-899X/274/1/012022

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

This study aims to explore changes of long-term fertilization on spore density and colonization of AMF (Arbuscular mycorrhizal fungi) under a 38-y long-term fertilization in a brown soil. Soil samples (0-20 cm,20-40cm,40-60cm)were taken from the six treatments of the long-term fertilization trial in October 2016:no fertilizer (CK), N1(mineral nitrogen fertilizer), N1P (mineral nitrogen and phosphate fertilizer), N1PK (mineral nitrogen, phosphate and potassic fertilizer), pig manure (M2), M2N1P (pig manure, mineral nitrogen andphosphate fertilizer).Spores were isolated from soils by wet sieving and sucrose density gradient centrifugation; mycorrhizal colonization levels were determined by the gridline intersect. The spore density was highest in the topsoils (0-20 cm), and was decreased with increasing of soil depth in each treatment. The spores density of M2N1P treatment was significantly higher than that of other treatments in each soil layer. Application of inorganic fertilizer (especially inorganic with organic fertilizer) can greatly improve the level of colonization. Our results suggested that long-term fertilization significantly affects spore density and colonization of AMF, however, spore density is not related to colonization rate.