Influence of Long-term Fertilization on AM Fungi Community Structures in a Brown Soil

2nd International Symposium on Resource Exploration and Environmental Science

生态环境科学

Guo, Jing^1,2 ; Luo, Peiyu^1,2 ; Han, Xiaori^1,2 ; Yang, Jinfeng^1,2 ; Li, Dongdong^1,2

College of Land and Environment, Shenyang Agricultural University, Shenyang, Liaoning

110866, China^1

National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, Shenyang, Liaoning

110866, China^2

关键词: Am fungus;    Arbuscular mycorrhizal;    Brown soil;    Cloning and sequencing;    Community structures;    Long-term fertilization;    Pig manures;    Soil sample;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/170/3/032106/pdf
DOI  :  10.1088/1755-1315/170/3/032106

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

This study aims to explore changes of community structures of AM (Arbuscular mycorrhizal) fungi after a long-term fertilization for 38 years in a brown soil. Soil samples (0-20 cm) were taken from six treatments in May 2016: (1) no fertilization (CK); (2) chemical N input (N); (3) chemical N and P input (NP); (4) chemical N, P and K input (NPK); (5) pig manure input (M); (6)pig manure, chemical N and P input (MNP). Community structures of AM (Arbuscular mycorrhizal) fungi were analyzed using PCR-DGGE, cloning and sequencing techniques. In seedling stage, the diversity of AM Fungi was the highest in non-fertilization treatment and the community structures of AM fungi was mainly divided into two groups.