Occurrence and detection method evaluation of group B streptococcus from prenatal vaginal specimen in Northwest China

Diagnostic Pathology

Qi Wang²  Hui Jia³  Peng Zhao¹  JunLan Yang³  Yun Xie³ 

[1] Colleges of Life Sciences, Northwest University, Xi’an 710069, Shaanxi, China;Department of Clinical Laboratory, the Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710004, Shaanxi, China;Department of Clinical Microbiology, Medical Laboratory Centre, Northwest Women’s and Children’s Hospital, Xi’an 710061, Shaanxi, China

关键词: Direct latex agglutination test;    PathoDxtra Strep Grouping Kit;    Carrot Broth;    Colonization rate;    Pregnant women;    Group B streptococcus;   

Others  :  1235685
DOI  :  10.1186/s13000-016-0463-9

 received in 2015-11-03, accepted in 2016-01-14,  发布年份 2016

【 摘 要 】

Sensitive and efficient detection of Group B Streptococcus (GBS) colonization in pregnant women is essential for prescription of prophylaxis at the time of delivery as GBS is an opportunistic pathogen known to cause infant mortality. In this report, two studies were conducted on the methods of GBS detection in Shaanxi province, China, a region lacking data for GBS detection and occurrence. For Study 1, 100 GBS culture-positive vaginal swabs were collected from 1,567 pregnant women for evaluation by direct latex agglutination test. In Study 2, 200 GBS vaginal swabs were evaluated by three culture methods (sheep blood agar (SBA), Columbia colistin-nalidixic agar (CNA), and selective carrot broth (SCB)) followed by analysis using a latex agglutination test. GBS was detected in 6.4 % of specimens in Study 1 and 10.5 % of specimens in Study 2. The results of the latex agglutination test in both studies were accurate with samples exhibiting high to moderate GBS growth, but the accuracy declined for samples with low GBS growth. The evaluation of culture methods for GBS detection revealed the sensitivity of SCB (95.2 %, p = 0.004) was significantly higher than that of the SBA medium (57.1 %). The sensitivity reported for SCB (95.2 %) was higher than CNA (76.0 %), but the difference was not statistically significant (p =0.078). These results indicate a selective broth, such as SCB, is ideal for accuracy at low growth levels, but a direct latex agglutination test could be used as an alternative for rapid detection of GBS in circumstances requiring immediate detection.

【 授权许可】

   
2016 Xie et al.

【 图 表 】

Fig. 1.

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