Antibacterial Activity of Ethyl Acetate the Extract of Noni Fruit (Morinda citrifolia L.) Against Bacterial Spoilage in Fish

International Conference On Food Science and Engineering 2016

Nugraheni, E.R.^1 ; Adriani, G.R.^2 ; Munawaroh, H.^3

Department of Pharmacy, Faculty of Mathematic and Natural Sciences, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta

57126, Indonesia^1

Departement of Biology, Faculty of Mathematic and Natural Sciences, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta

57126, Indonesia^2

Inorganic Materials Research Group, Faculty of Mathematics and Natural Sciences, Sebelas Maret University, Jl. Ir. Sutami No. 36A, Surakarta, Indonesia^3

关键词: Anti-bacterial activity;    Antibacterial compounds;    Enterobacter aerogenes;    Flavonoids compounds;    Inhibition activity;    Minimum bactericidal concentrations;    Minimum inhibitor concentrations;    Pseudomonas aeruginosa;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/193/1/012019/pdf
DOI  :  10.1088/1757-899X/193/1/012019

来源: IOP

【 摘 要 】

Noni fruit (Morinda citrifolia L.) contains compounds that have potential as antibacterial agent. Antibacterial compounds produced noni fruit (M. citrifolia L.) can inhibit bacterial growth. This study was conducted to test the antibacterial activity of ethyl acetate extract of noni fruit (M. citrifolia L.) against spoilage bacterial in fish. Pseudomonas aeruginosa, Bacillus cereus, Escherichia coli, Klebsiella oxytoca, and Enterobacter aerogenes isolates and examine antibacterial phytochemical profile. Extraction of noni compounds was done by maceration, followed by partition with ethyl acetate to obtain the soluble and insoluble ethyl acetate fraction. Previews result show that the ethyl acetate extract had very strong activity. Extraction process continued by separation and isolation used preparative thin layer chromatography method, so that obtained five isolates and mark them as A, B, C, D and E. Antibacterial activity assay performed on isolates A, B, C, D, and E with 20 and 30% concentration. The test results showed that isolates A could not be inhibit the growth of bacteria, isolates B, C, D, and E has antibacterial activity with weak to strong inhibition. Isolate B had the greatest inhibition activity against the B. cereus, whereas isolates E had the greatest inhibition activity against P. aeroginosa. MIC (Minimum Inhibitor Concentration) and MBC (Minimum Bactericidal Concentration) test result showed that MIC and MBC values could not be determined. Analysis of compounds by TLC showed that isolate B suspected contains coumarin or flavonoids compounds that have antibacterial activity.