The spread of bla OXA-48 and bla OXA-244 carbapenemase genes among Klebsiella pneumoniae, Proteus mirabilis and Enterobacter spp. isolated in Moscow, Russia

Annals of General Psychiatry

Nadezhda K. Fursova²  Eugeny I. Astashkin²  Anastasia I. Knyazeva²  Nikolay N. Kartsev²  Ekaterina S. Leonova²  Olga N. Ershova¹  Irina A. Alexandrova¹  Natalia V. Kurdyumova¹  Svetlana Yu. Sazikina¹  Nikolay V. Volozhantsev²  Edward A. Svetoch²  Ivan A. Dyatlov² 

[1] The Burdenko Neurosurgery Institute, Moscow 125047, Russia

[2] State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, 142279, Russia

关键词: Horizontal gene transfer;    Antibacterial resistance;    Hospital pathogens;    OXA-48-like carbapenemase;    Enterobacter cloacae;    Enterobacter aerogenes;    Proteus mirabilis;    Klebsiella pneumoniae;    Enterobacteriaceae;   

Others  :  1231018
DOI  :  10.1186/s12941-015-0108-y

 received in 2015-07-21, accepted in 2015-10-15,  发布年份 2015

【 摘 要 】

Background

The spread of carbapenemase-producing Enterobacteriaceae (CPE) is a great problem of healthcare worldwide. Study of the spread for bla_OXA-48-likegenes coding epidemically significant carbapenemases among hospital pathogens is important for the regional and global epidemiology of antimicrobial resistance.

Methods

Antibacterial resistant isolates of Klebsiella pneumoniae (n = 95) from 54 patients, P. mirabilis (n = 32) from 20 patients, Enterobacter aerogenes (n = 6) from four patients, and Enterobacter cloacae (n = 4) from four patients were collected from January, 2013 to October, 2014 in neurosurgical intensive care unit (ICU) of the Burdenko Neurosurgery Institute, Moscow. Characteristics of the isolates were done using susceptibility tests, PCR detection of the resistance genes, genotyping, conjugation, DNA sequencing, and bioinformatic analysis.

Results

Major strains under study were multi drug resistant (MDR), resistant to three or more functional classes of drugs simultaneously—98.9 % K. pneumoniae, 100 % P. mirabilis, one E. aerogenes isolate, and one E. cloacae isolate. Molecular-genetic mechanism of MDR in K. pneumoniae and P. mirabilis isolates were based on carrying of epidemic extended-spectrum beta-lactamase bla_CTX-M-15gene (87.2 and 90.6 % accordingly), carbapenemase bla_OXA-48-likegene (55.3 and 23.3 % accordingly), and class 1 (54.8 and 31.3 % accordingly) and class 2 (90.6 % P. mirabilis) integrons. The bla_OXA-48-like -positive K. pneumoniae were collected during whole two-year surveillance period, while P. mirabilis and Enterobacter spp. carrying bla_OXA-48-likegenes were detected only after four and 18 months after the research start, respectively. The bla_OXA-48-likegene acquisition was shown for P. mirabilis isolates collected from five patients and for E. cloacae isolate collected from one patient during their stay in the ICU, presumably from bla_OXA-48-like -positive K. pneumoniae. The source of the bla_OXA-244gene acquired by E. aerogenes isolates and the time of this event were not recognized.

Conclusions

The expanding of CPE in the surveyed ICU was associated with the spread of bla_OXA-48and bla_OXA-244carbapenemase genes documented not only among K. pneumoniae, well-known bacterial host for such genes, but among P. mirabilis, E. aerogenes, and E. cloacae.

【 授权许可】

   
2015 Fursova et al.

【 图 表 】

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