Comparison on mechanical properties of single layered and bilayered chitosan-gelatin coated porous hydroxyapatite scaffold prepared through freeze drying method

11th Joint Conference on Chemistry in Conjunction with the 4th Regional Biomaterials Scientific Meeting

材料科学;化学;生物科学

Effendi, M.D.^1 ; Gustiono, D.^1 ; Lukmana^1 ; Ayu, D.^1 ; Kurniawati, F.^1

Agency for the Assessment and Application of Technolgy, Serpong, Indonesia^1

关键词: Chitosan gelatins;    Coating procedures;    Coating structures;    Freeze drying method;    Impregnation methods;    Organic components;    Polyurethane Foam;    Porous hydroxyapatite;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/172/1/012031/pdf
DOI  :  10.1088/1757-899X/172/1/012031

来源: IOP

【 摘 要 】

Biopolymer coated porous hydroxyapatite (HA) scaffolds were prepared for tissue engineering trough freeze drying method and impregnation. in this study, to mimic the mineral and organic component of natural bone, synthetic hydroxapatite (HA) scaffolds coated by polymer were prepared. Highly porous Hap scaffolds, fabricated by synthetic HA impregnation method on polyurethane foam, were coated with polymer coating solution, consisting of chitosan, Gelatin, and bilayered chitosan-gelatin prepared by aging and impregnating technique. For the purpose of comparison, The bare scaffolds without polymer coating layer were investigated. The Bare scaffolds were highly porous and interconnected with a pore size of around 150 μm-714 μm, has porosity at around 67,7% -85,7%, and has mechanical strength at around 0.06 Mpa - 0.071 Mpa, which is suitable for osteoblast cell Proliferation. Chitosan coated porous HA scaffold and gelatin coated porous HA scaffold had mechanical strength at around 0.81-0.85 Mpa, and 1.32-1.34 Mpa, respectively, with weight ratio of biopolymer and Hap was around 18%-22%. To compare these results, the coating on the bare scaffold with gelatin and chitosan had been conducted. Based on the result of FTIR, it could be concluded that coating procedure applied on porous hydroxy apatite (HA) coated by gelatin, chitosan coated HA scaffold, and bilayered Gelatin-chitosan coated porous HA scaffold, confirming that for allsampleshad no significant chemical effect on the coating structure. The compressive strength of bilayered Gelatin-chitosan coated HA scaffold had middle values between the rest, at around 1,06-1.2 Mpa for the samples at the same weight ratio of biopolymer: HA (around 18% - 22%). These results also confirming that coating by gelatin on porous hydroxyapatite was highest compresive strength and can be applied to improve mechanical properties of porous hydroxyapatite bare scaffold.