Influence of thickness of alkyl-silane coupling agent coating on separation of small DNA fragments in capillary gel electrophoresis

2017 3rd International Conference on Applied Materials and Manufacturing Technology

Nakazumi, T.^1 ; Hara, Y.^1

Research Institute for Sustainable Chemistry, ISC, National Institute of Advanced Industrial Science and Technology, AIST, Central 5-2, 1-1-1 Higashi, Tsukuba

305-8565, Japan^1

关键词: Capillary gel electrophoresis;    Coating procedures;    DNA fragment;    Electroosmotic flow;    Fused-silica capillaries;    Molecular length;    Silane coupling agent;    Total length;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/242/1/012034/pdf
DOI  :  10.1088/1757-899X/242/1/012034

来源: IOP

【 摘 要 】

To simplify the process of coating capillaries with fused silica, we herein set out to develop a one-step procedure for coating capillaries to prevent electro-osmotic flow (EOF) during the separation of small DNA fragments. We selected a short capillary (total length = 15 cm; effective length = 7.5 cm) for use in a compact capillary gel electrophoresis (CGE) system. To develop a one-step coating procedure, we employed alkyltrimethoxysilane agents because they are cheap and can be easily acquired, in contrast to polyethylene glycol (PEG) silane coupling agents. We examined a 100-bp DNA Ladder sample using fused silica capillaries, which were coated with alkyltrimethoxysilane agents of five different molecular lengths (C4, C6, C8, C12, and C16). We found that a fused-silica capillary with C8 alkyltrimethoxysilane is optimal for separating small DNA samples.