Development of high performance liquid chromatography method for miconazole analysis in powder sample

11th Joint Conference on Chemistry in Conjunction with the 4th Regional Biomaterials Scientific Meeting

材料科学;化学;生物科学

Hermawan, D.^1 ; Suwandri^1 ; Sulaeman, U.^1 ; Istiqomah, A.^1 ; Aboul-Enein, H.Y.^2

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Jenderal Soedirman University, Purwokerto, Indonesia^1

Department of Pharmaceutical and Medicinal Chemistry, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki, Cairo, Egypt^2

关键词: Antifungal drugs;    Calibration graphs;    High reproducibility;    High sensitivity;    Limit of detection;    Limit of quantitations;    Mobile-phase composition;    Powder samples;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/172/1/012011/pdf
DOI  :  10.1088/1757-899X/172/1/012011

来源: IOP

【 摘 要 】

A simple high performance liquid chromatography (HPLC) method has been developed in this study for the analysis of miconazole, an antifungal drug, in powder sample. The optimized HPLC system using C8column was achieved using mobile phase composition containing methanol:water (85:15, v/v), a flow rate of 0.8 mL/min, and UV detection at 220 nm. The calibration graph was linear in the range from 10 to 50 mg/L with r2of 0.9983. The limit of detection (LOD) and limit of quantitation (LOQ) obtained were 2.24 mg/L and 7.47 mg/L, respectively. The present HPLC method is applicable for the determination of miconazole in the powder sample with a recovery of 101.28 % (RSD = 0.96%, n = 3). The developed HPLC method provides short analysis time, high reproducibility and high sensitivity.