Multi-walled carbon nanotubes: biodegradation by gastric agents in vitro and effect on murine intestinal system

3rd International Youth Conference on Interdisciplinary Problems of Nanotechnology, Biomedicine and Nanotoxicology

物理学;医药卫生;生态环境科学

Masyutin, A.^1 ; Erokhina, M.^1 ; Sychevskaya, K.^1 ; Gusev, A.^2 ; Vasyukova, I.^2 ; Smirnova, E.^1 ; Onishchenko, G.^1

Department of Cell Biology and Histology, Biology Faculty, Lomonosov Moscow State University, Leninskie Gory 1/12, Moscow

119234, Russia^1

NanoCenter of Nanotechnologies and Nanomaterials, Tambov Derzhavin State University, Zashchitnii pereulok 7, Tambov, Russia^2

关键词: Analytical electron microscopy;    Fibrous carbons;    Food industries;    Gastric juice;    Gastrointestinal tract;    Living organisms;    Oral administration;    Small intestine;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/98/1/012008/pdf
DOI  :  10.1088/1757-899X/98/1/012008

来源: IOP

【 摘 要 】

One of the main questions limiting application of fibrous carbon nanomaterials (CNM) in medicine and food industry concerns presumptive degradation of CNM in living organisms. In this study, we have investigated biodegradation of multi-walled carbon nanotubes (MWCNTs) by gastric agents in vitro and influence of ingested MWCNTs on murine intestine. Using scanning, conventional transmission and analytical electron microscopy, we demonstrated that industrial MWCNTs treated in vitro by 0.1 M hydrochloric acid (pH=1) and gastric juice (pH=2-3) isolated from murine stomach, are subjected to incomplete degradation. After 30 days of oral administration to experimental mice, we did find MWCNTs in the cells of small intestine, and it may indicate that agglomerates of MWCNTs do not penetrate into colon epithelia and do not accumulate in enterocytes. However, we observed local areas of necrotic damages of intestinal villi. It seems likely, therefore, that MWCNTs end up leaving gastrointestinal tract by excretion with the feces. Our results suggest that MWCNTs do not undergo complete degradation in gastrointestinal tract of mice, and passing through non-degraded particles may negatively affect intestinal system.