Genomic validation of PB 260 clone of rubber (Hevea brasiliensis) at Cikumpay Plantation by SSR marker

International Symposium on Bioinformatics, Chemometrics and Metabolomics

生物科学;化学

Royani, J.I.^1 ; Safarrida, A.^1 ; Rachmawati, I.^1 ; Khairiyah, H.^1 ; Mustika, I.P.^1 ; Suyono, A.^1 ; Rudiyana, Y.^1 ; Kubil^1 ; Nurjaya^1 ; Arianto, A.^1

Badan Pengkajian Dan Penerapan Teknologi, Gd LAPTIAB 610, Kawasan PUSPIPTEK, Serpong Tangerang Selatan Banten

15314, Indonesia^1

关键词: Ctab modifications;    Hevea brasiliensis;    In-vitro;    Mother plant;    Rubber tree;    Seed germination;    SSR markers;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/835/1/012009/pdf
DOI  :  10.1088/1742-6596/835/1/012009

学科分类：生物科学（综合）

来源: IOP

【 摘 要 】

Rubber from Hevea brasiliensis is the only commercial natural rubber in the world. Propagation of rubber trees usually done by grafting and seed germination. BPPT had been producing rubber tree by in vitro technique with embryo somatic methods. Validation of mother plant for in vitro propagation is important to compare between mother plant and propagated plants. The aim for this research was to validation of PB 260 clone that planted at Cikumpay Plantation by SSR marker. Sampling of 10 rubber leaves were done at Cikumpay Plantation based on GPS position from the area of PB 260 clone. Rubber leaves were isolated with CTAB modification method to obtained DNA. Four of SSR primers from rubber, i.e.: hmac 4, hmac 5, hmct 1, and hmct 5, were used as primers to amplification of rubber DNA. The result showed that no band that different from 10 rubber of PB 260 clone at Cikumpay Plantation. This research will continue to compare genomic validation between mother plant and propagated plants that had been produced from BPPT.