Amplification of 5 Accessions of DNA Binahong (Anredera Cordifolia (Ten.) Steenis) By Inter Simple Sequence Repeats as Tool for Molecular Marker

2nd International Conference on Biosciences

Royani, J.I.^1 ; Maulana, N.A.^2 ; Dasumiati^2

Badan Pengkajian Dan Penerapan Teknologi, Gd. Laptiab 610, Kawasan PUSPIPTEK Serpong Tangerang

15314, Indonesia^1

Jurusan Biologi, Fakultas Sains Dan Teknologi, Universitas Islam Negeri Syarif Hidayatullah, Jl. Ir. H. Juanda 95, Ciputat Tangerang Selatan, Indonesia^2

关键词: Anredera cordifolia;    Binahong;    Ctab modifications;    Inter-simple sequence repeats;    ISSR primers;    Multiple function;    Polymorphic bands;    Traditional medicines;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/197/1/012047/pdf
DOI  :  10.1088/1755-1315/197/1/012047

来源: IOP

【 摘 要 】

Binahong or Anredera cordifolia (Ten.) Steenis is a plant with multiple functions for medicine. It was used as anti-hipercholesterolemia, anti-hyperlipidemia, wound healing, antioxidant, analgesic, skin diseases, anti-cancer and anti-diabetic. Binahong origin from Paraguay to Southern Brazil and Northern Argentina but spreads into Africa, Europe, Australia, and Asia, including Indonesia. In Indonesia, binahong distributed to almost in all island that used for traditional medicines. The aim of this research was to amplify DNA from 5 accessions of binahong with 15 Inter-Simple Sequence Repeats (ISSR) primers for the screening of primers. DNA of 5 accessions of Binahong from Tagari-Toraja, Solok-Sumatera Barat, Rante-Langda, Petulu-Bali, and Makale-Toraja were isolated by CTAB modification methods and were amplified by 15 ISSR primers. Amplification was done at PCR machine with the condition: pre-denaturation at 94°C for 5 minutes, denaturation at 94°C for 1 minute, annealing at 45 seconds, elongation at 72°C for 2 minutes, and final elongation at 72°C for 5 minutes for 40 cycles. The results showed that 8 primers were able to amplification of DNA binahong, while 7 primers failed to amplification. The eight of ISSR primers produced polymorphic bands and those are SBLT2, SBLT3, SBLT4, SBLT6, SBLT8, SBLT13, SBLT14 and SBLT15 primers.