Use of optogenetic technology in cell culture models

3rd International School and Conference on Optoelectronics, Photonics, Engineering and Nanostructures (Saint Petersburg OPEN 2016)

Erofeev, A.I.^1 ; Zakharova, O.A.^1 ; Matveev, M.V.^1 ; Terekhin, S.G.^1 ; Bezprozvanny, I.B.^1,2 ; Vlasova, O.L.^1

Laboratory of Molecular Neurodegeneration, Peter the Great Polytechnic University, Saint-Petersburg

195251, Russia^1

Department of Physiology, University of Texas Southwestern Medical Center, Dallas

TX, United States^2

关键词: Cell culture models;    Cellular membranes;    HEK-293;    Hippocampal neurons;    Light stimulation;    Physiological properties;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/741/1/012067/pdf
DOI  :  10.1088/1742-6596/741/1/012067

来源: IOP

【 摘 要 】

Optogenetic is a powerful method that allows to modulate cellular physiological properties. In our article, we demonstrate changes of electrical properties of cellular membranes on HEK-293T and hippocampal neurons transfected with channelrhodopsins and halorhodopsins induced by blue and orange light stimulation.