Three-Photon Luminescence of Gold Nanorods Excited by 1040 nm Femtosecond Laser for High Contrast Tissue and In Vivo Imaging

5th International Conference on Advances in Optoelectronics and Micro/Nano-optics

Wang, Shaowei^1 ; Zhao, Xinyuan^2 ; Zhang, Hequn^1 ; Cai, Fuhong^1,3 ; Qian, Jun^1

State Key Laboratory of Modem Optical Instrumentations, Centre for Optical and Electromagnetic Research, Zhejiang University, Hangzhou

310058, China^1

Bioelectromagnetics Laboratory, School of Medicine, Zhejiang University, Hangzhou

310058, China^2

Suzhou WiHealth Information Technology Co. Ltd, Changshu

215500, China^3

关键词: Autofluorescence;    Contrast agent;    Gold nanorods (GNRs);    In-Vivo imaging;    Localized surface plasmon resonance;    Multiphoton luminescence;    Optical window;    Organic chromophores;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/680/1/012013/pdf
DOI  :  10.1088/1742-6596/680/1/012013

来源: IOP

【 摘 要 】

Gold Nanorods (GNRs) with tunable aspect ratios can strongly absorb and scatter light in the NIR region due to their localized surface plasmon resonance (LSPR) property, and have been demonstrated to exhibit strong plasmon enhanced multiphoton luminescence (MPL) with brightness many times stronger than the conventional organic chromophores. In this study, we synthesized GNRs with longitudinal LSPR peak at 1036 nm to match our home-built light source 1040 nm femtosecond laser, which locates in the "optical window" where the tissue absorbs relatively little light. PEGylated GNRs with great biocompatibility were intravenously injected through the tail vein into mice. Excited by 1040 nm laser, the GNRs exhibit bright three-photon luminescence (3PL) signals while circulating in the blood vessels. The use of GNRs as bright contrast agents for 3PL imaging of mouse ear blood vessels in vivo was demonstrated. And GNRs targeted in tissues can be excited by 1040 nm laser and could be clearly visualized with no autofluorescence background. These results indicated that 3PL of GNRs is very promising for deep in vivo bioimaging and assessing the distribution of GNRs in tissues with high contrast.