The impact of instilled carbide nanoparticles on rat lungs: an in vivo perspective on acute intratracheal instillation

4th International Conference on Safe Production and Use of Nanomaterials

Lozano, O.^1 ; Lison, D.^2 ; Escamilla-Rivera, V.^3 ; Mejía, J.^1 ; Toussaint, O.^4 ; Dogné, J.M.^5 ; Lucas, S.^1

Research Centre for the Physics of Matter and Radiation (PMR), Namur Nanosafety Center (NNC), NAmur Research Institute for LIfe Sciences (NARILIS), University of Namur (UNamur), Namur, Belgium^1

Louvain Centre for Toxicology and Applied Pharmacology, Université Catholique de Louvain, Brussels, Belgium^2

Department of Toxicology, Center of Research and Advanced Studies of IPN (CINVESTAV-IPN), Mexico City, D.F., Mexico^3

Laboratory of Biochemistry and Cellular Biology (URBC), Namur Nanosafety Center (NNC), NAmur Research Institute for LIfe Sciences (NARILIS), University of Namur (UNamur), Namur, Belgium^4

Department of Pharmacy, Namur Nanosafety Center (NNC), NAmur Research Institute for LIfe Sciences (NARILIS), University of Namur (UNamur), Namur, Belgium^5

关键词: Carbide nanoparticles;    Differentiated cells;    Female Wistar rats;    Genotoxicities;    Inflammatory response;    Intratracheal instillation;    Lactate dehydrogenase activities;    Protein concentrations;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/617/1/012017/pdf
DOI  :  10.1088/1742-6596/617/1/012017

来源: IOP

【 摘 要 】

In order to study a scenario of acute high concentration exposure via the pulmonary pathway of silicon carbide and titanium carbide nanoparticles, female Wistar rats were administered by intratracheal instillation doses of 0.5 and 5 mg/rat of each nanomaterial. Inflammatory parameters were studied: protein concentration, lactate dehydrogenase activity, total cell count and differentiated cell count (macrophages, neutrophils, oesonophils, lymphocytes). The genotoxicity potential was assessed by the formation of micronuclei from pneumocytes type II. It was found that silicon carbide nanoparticles induce an inflammatory response and a dose dependent genotoxicity, although the genotoxicity levels are comparably lower to the inflammatory response.