Nanoparticle exposure biomonitoring: exposure/effect indicator development approaches

4th International Conference on Safe Production and Use of Nanomaterials

Marie-Desvergne, C.^1 ; Dubosson, M.^1 ; Lacombe, M.^2 ; Brun, V.^2 ; Mossuz, V.^1

Univ. Grenoble Alpes, CEA, Nanosafety Platform, Medical Biology Laboratory (LBM), 17 rue des Martyrs, Grenoble

F-38054, France^1

Univ. Grenoble Alpes, CEA, INSERM, IRTSV, U1038 Exploring the Dynamics of Proteomes Laboratory (EDyP), 17 rue des Martyrs, Grenoble

F-38054, France^2

关键词: Analytical characterization;    Engineered nanoparticles;    Exhaled breath condensate;    Field flow fractionation;    Indicator development;    Innovative techniques;    Nanoparticle exposures;    Occupational health;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/617/1/012005/pdf
DOI  :  10.1088/1742-6596/617/1/012005

来源: IOP

【 摘 要 】

The use of engineered nanoparticles (NP) is more and more widespread in various industrial sectors. The inhalation route of exposure is a matter of concern (adverse effects of air pollution by ultrafine particles and asbestos). No NP biomonitoring recommendations or standards are available so far. The LBM laboratory is currently studying several approaches to develop bioindicators for occupational health applications. As regards exposure indicators, new tools are being implemented to assess potentially inhaled NP in non-invasive respiratory sampling (nasal sampling and exhaled breath condensates (EBC)). Diverse NP analytical characterization methods are used (ICP-MS, dynamic light scattering and electron microscopy coupled to energy-dispersive X-ray analysis). As regards effect indicators, a methodology has been developed to assess a range of 29 cytokines in EBCs (potential respiratory inflammation due to NP exposure). Secondly, collaboration between the LBM laboratory and the EDyp team has allowed the EBC proteome to be characterized by means of an LC-MS/MS process. These projects are expected to facilitate the development of individual NP exposure biomonitoring tools and the analysis of early potential impacts on health. Innovative techniques such as field-flow fractionation combined with ICP-MS and single particle-ICPMS are currently being explored. These tools are directly intended to assist occupational physicians in the identification of exposure situations.