The use of transmission electron microscopy in the quantification of nanoparticle dose

Electron Microscopy and Analysis Group Conference 2013

Hondow, N.^1 ; Brydson, R.^1 ; Brown, A.^1

Institute for Materials Research, School of Process, Environmental and Materials Engineering, University of Leeds, Leeds LS2 9JT, United Kingdom^1

关键词: Biological impacts;    Biomedical imaging;    Clinical medicine;    Contrast agent;    Dose distributions;    Quantitative description;    Thin cells;    Whole cell;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/522/1/012055/pdf
DOI  :  10.1088/1742-6596/522/1/012055

来源: IOP

【 摘 要 】

There are an increasing number of potential applications for nanoparticles in clinical medicine, including targeted drug delivery and contrast agents for biomedical imaging. Current in vitro studies are concerned with the biological impact of nanoparticles, with electron microscopy commonly employed to image their intracellular location. It is critical to quantify the absolute nanoparticle dose internalized by cells in a given exposure, and to understand the factors which affect this. In this work we are aiming to develop a full quantitative description of quantum dot uptake by an in vitro cell line. Transmission electron microscopy of thin cell sections provides the location and number of cellular vesicles per 2-D cell slice plus the number of quantum dots per vesicle. These results can then be correlated to other techniques to quantify the internalized nanoparticle dose distribution for whole cells.